1axh
From Proteopedia
(New page: 200px<br /><applet load="1axh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1axh" /> '''ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUS...) |
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| - | [[Image:1axh.gif|left|200px]]<br /><applet load="1axh" size=" | + | [[Image:1axh.gif|left|200px]]<br /><applet load="1axh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1axh" /> | caption="1axh" /> | ||
'''ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES'''<br /> | '''ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A family of potent insecticidal toxins has recently been isolated from the | + | A family of potent insecticidal toxins has recently been isolated from the venom of Australian funnel web spiders. Among these is the 37-residue peptide omega-atracotoxin-HV1 (omega-ACTX-HV1) from Hadronyche versuta. We have chemically synthesized and folded omega-ACTX-HV1, shown that it is neurotoxic, ascertained its disulphide bonding pattern, and determined its three-dimensional solution structure using NMR spectroscopy. The structure consists of a solvent-accessible beta-hairpin protruding from a disulphide-bonded globular core comprising four beta-turns. The three intramolecular disulphide bonds from a cystine knot motif similar to that seen in several other neurotoxic peptides. Despite limited sequence identity, omega-ACTX-HV1 displays significant structural homology with the omega-agatoxins and omega-conotoxins, both of which are vertebrate calcium channel antagonists; however, in contrast with these toxins, we show that omega-ACTX-HV1 inhibits insect, but not mammalian, voltage-gated calcium channel currents. |
==About this Structure== | ==About this Structure== | ||
| - | 1AXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full crystallographic information is available from [http:// | + | 1AXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AXH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Hadronyche versuta]] | [[Category: Hadronyche versuta]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Donoghue, S | + | [[Category: Donoghue, S I.O.]] |
| - | [[Category: Fletcher, J | + | [[Category: Fletcher, J I.]] |
| - | [[Category: King, G | + | [[Category: King, G F.]] |
[[Category: Nilges, M.]] | [[Category: Nilges, M.]] | ||
[[Category: cystine knot]] | [[Category: cystine knot]] | ||
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[[Category: neurotoxin]] | [[Category: neurotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:49:16 2008'' |
Revision as of 09:49, 21 February 2008
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ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES
Overview
A family of potent insecticidal toxins has recently been isolated from the venom of Australian funnel web spiders. Among these is the 37-residue peptide omega-atracotoxin-HV1 (omega-ACTX-HV1) from Hadronyche versuta. We have chemically synthesized and folded omega-ACTX-HV1, shown that it is neurotoxic, ascertained its disulphide bonding pattern, and determined its three-dimensional solution structure using NMR spectroscopy. The structure consists of a solvent-accessible beta-hairpin protruding from a disulphide-bonded globular core comprising four beta-turns. The three intramolecular disulphide bonds from a cystine knot motif similar to that seen in several other neurotoxic peptides. Despite limited sequence identity, omega-ACTX-HV1 displays significant structural homology with the omega-agatoxins and omega-conotoxins, both of which are vertebrate calcium channel antagonists; however, in contrast with these toxins, we show that omega-ACTX-HV1 inhibits insect, but not mammalian, voltage-gated calcium channel currents.
About this Structure
1AXH is a Single protein structure of sequence from Hadronyche versuta. Full crystallographic information is available from OCA.
Reference
The structure of a novel insecticidal neurotoxin, omega-atracotoxin-HV1, from the venom of an Australian funnel web spider., Fletcher JI, Smith R, O'Donoghue SI, Nilges M, Connor M, Howden ME, Christie MJ, King GF, Nat Struct Biol. 1997 Jul;4(7):559-66. PMID:9228949
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