4cms

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[[Image:4cms.gif|left|200px]]
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[[Image:4cms.png|left|200px]]
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==Overview==
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{{ABSTRACT_1942052}}
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The structure of calf chymosin (EC 3.4.23.3), the aspartic proteinase from the gastric mucosa, was solved using the technique of molecular replacement. We describe the use of different search models based on distantly related fungal aspartic proteinases and investigate the effect of using only structurally conserved regions. The structure has been refined to a crystallographic R-factor of 17% at 2.2 A resolution with an estimated co-ordinate error of 0.21 A. In all, 136 water molecules have been located of which eight are internal. The structure of chymosin resembles that of pepsin and other aspartic proteinases. However, there is a considerable rearrangement of the active-site "flap" and, in particular, Tyr75 (pepsin numbering), which forms part of the specificity pockets S1 and S1'. This is probably a consequence of crystal packing. Electrostatic interactions on the edge of the substrate binding cleft appear to account for the restricted proteolysis of the natural substrate kappa-casein by chymosin. The local environment of invariant residues is examined, showing that structural constraints and side-chain hydrogen bonding can play an important role in the conservation of particular amino acids.
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==About this Structure==
==About this Structure==
4CMS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The following page contains interesting information on the relation of 4CMS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb12_1.html Pepsin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CMS OCA].
4CMS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The following page contains interesting information on the relation of 4CMS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb12_1.html Pepsin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CMS OCA].
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==Reference==
 
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X-ray analyses of aspartic proteinases. IV. Structure and refinement at 2.2 A resolution of bovine chymosin., Newman M, Safro M, Frazao C, Khan G, Zdanov A, Tickle IJ, Blundell TL, Andreeva N, J Mol Biol. 1991 Oct 20;221(4):1295-309. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1942052 1942052]
 
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Chymosin]]
[[Category: Chymosin]]
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[[Category: Tickle, I J.]]
[[Category: Tickle, I J.]]
[[Category: Zdanov, A.]]
[[Category: Zdanov, A.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:22:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:28:59 2008''

Revision as of 15:29, 26 June 2008

Image:4cms.png

Template:STRUCTURE 4cms

X-RAY ANALYSES OF ASPARTIC PROTEINASES IV. STRUCTURE AND REFINEMENT AT 2.2 ANGSTROMS RESOLUTION OF BOVINE CHYMOSIN


Template:ABSTRACT 1942052

About this Structure

4CMS is a Single protein structure of sequence from Bos taurus. The following page contains interesting information on the relation of 4CMS with [Pepsin]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:28:59 2008

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