This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4ktq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:4ktq.gif|left|200px]]
+
{{Seed}}
 +
[[Image:4ktq.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_4ktq| PDB=4ktq | SCENE= }}
{{STRUCTURE_4ktq| PDB=4ktq | SCENE= }}
-
'''BINARY COMPLEX OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM T. AQUATICUS BOUND TO A PRIMER/TEMPLATE DNA'''
+
===BINARY COMPLEX OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM T. AQUATICUS BOUND TO A PRIMER/TEMPLATE DNA===
-
==Overview==
+
<!--
-
The crystal structures of two ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I (Klentaq1) with a primer/template DNA and dideoxycytidine triphosphate, and that of a binary complex of the same enzyme with a primer/template DNA, were determined to a resolution of 2.3, 2.3 and 2.5 A, respectively. One ternary complex structure differs markedly from the two other structures by a large reorientation of the tip of the fingers domain. This structure, designated 'closed', represents the ternary polymerase complex caught in the act of incorporating a nucleotide. In the two other structures, the tip of the fingers domain is rotated outward by 46 degrees ('open') in an orientation similar to that of the apo form of Klentaq1. These structures provide the first direct evidence in DNA polymerase I enzymes of a large conformational change responsible for assembling an active ternary complex.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9857206}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9857206 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9857206}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Large fragement of taq dna polymerase i]]
[[Category: Large fragement of taq dna polymerase i]]
[[Category: Protein/dna]]
[[Category: Protein/dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:26:04 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:42:45 2008''

Revision as of 10:42, 3 July 2008

Image:4ktq.png

Template:STRUCTURE 4ktq

BINARY COMPLEX OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM T. AQUATICUS BOUND TO A PRIMER/TEMPLATE DNA

Template:ABSTRACT PUBMED 9857206

About this Structure

4KTQ is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation., Li Y, Korolev S, Waksman G, EMBO J. 1998 Dec 15;17(24):7514-25. PMID:9857206

Page seeded by OCA on Thu Jul 3 13:42:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ktq"
Personal tools