1azf
From Proteopedia
(New page: 200px<br /><applet load="1azf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1azf, resolution 1.8Å" /> '''CHICKEN EGG WHITE LYS...) |
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| - | [[Image:1azf.gif|left|200px]]<br /><applet load="1azf" size=" | + | [[Image:1azf.gif|left|200px]]<br /><applet load="1azf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1azf, resolution 1.8Å" /> | caption="1azf, resolution 1.8Å" /> | ||
'''CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION'''<br /> | '''CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Anions have been shown to play a dominant role in the crystallization of | + | Anions have been shown to play a dominant role in the crystallization of chicken egg-white lysozyme from salt solutions. Previous studies employing X-ray crystallography have found one chloride ion binding site in the tetragonal crystal form of the protein and four nitrate ion binding sites in the monoclinic form. In this study the anion positions in the tetragonal form were determined from the difference Fourier map obtained from lysozyme crystals grown in bromide and chloride solutions. Five possible anion-binding sites were found in this manner. Some of these sites were in pockets containing basic residues while others were near neutral, but polar, residues. The sole chloride ion binding site found in previous studies was confirmed, while four further sites were found which corresponded to the four binding sites found for nitrate ions in monoclinic crystals. The study suggests that most of the anion-binding sites in lysozyme remain unchanged even when different anions and different crystal forms of lysozyme are employed. |
==About this Structure== | ==About this Structure== | ||
| - | 1AZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with BR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1AZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZF OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Forsythe, E | + | [[Category: Forsythe, E L.]] |
[[Category: Lim, K.]] | [[Category: Lim, K.]] | ||
[[Category: Nadarajah, A.]] | [[Category: Nadarajah, A.]] | ||
| - | [[Category: Pusey, M | + | [[Category: Pusey, M L.]] |
[[Category: BR]] | [[Category: BR]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: o-glycosyl hydrolase]] | [[Category: o-glycosyl hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:49:50 2008'' |
Revision as of 09:49, 21 February 2008
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CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION
Overview
Anions have been shown to play a dominant role in the crystallization of chicken egg-white lysozyme from salt solutions. Previous studies employing X-ray crystallography have found one chloride ion binding site in the tetragonal crystal form of the protein and four nitrate ion binding sites in the monoclinic form. In this study the anion positions in the tetragonal form were determined from the difference Fourier map obtained from lysozyme crystals grown in bromide and chloride solutions. Five possible anion-binding sites were found in this manner. Some of these sites were in pockets containing basic residues while others were near neutral, but polar, residues. The sole chloride ion binding site found in previous studies was confirmed, while four further sites were found which corresponded to the four binding sites found for nitrate ions in monoclinic crystals. The study suggests that most of the anion-binding sites in lysozyme remain unchanged even when different anions and different crystal forms of lysozyme are employed.
About this Structure
1AZF is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Locations of bromide ions in tetragonal lysozyme crystals., Lim K, Nadarajah A, Forsythe EL, Pusey ML, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):899-904. PMID:9757106
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