4rnp

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{{STRUCTURE_4rnp| PDB=4rnp | SCENE= }}
{{STRUCTURE_4rnp| PDB=4rnp | SCENE= }}
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'''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''
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===BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY===
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==Overview==
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The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.
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(as it appears on PubMed at http://www.pubmed.gov), where 7688864 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7688864}}
==About this Structure==
==About this Structure==
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[[Category: Rna polymerase]]
[[Category: Rna polymerase]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:49:20 2008''

Revision as of 10:49, 3 July 2008

Image:4rnp.png

Template:STRUCTURE 4rnp

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

Template:ABSTRACT PUBMED 7688864

About this Structure

4RNP is a Single protein structure of sequence from Enterobacteria phage t7. This structure supersedes the now removed PDB entries and 2rnp. Full crystallographic information is available from OCA.

Reference

Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution., Sousa R, Chung YJ, Rose JP, Wang BC, Nature. 1993 Aug 12;364(6438):593-9. PMID:7688864

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