5nuc

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[[Image:5nuc.gif|left|200px]]
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{{Seed}}
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[[Image:5nuc.png|left|200px]]
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{{STRUCTURE_5nuc| PDB=5nuc | SCENE= }}
{{STRUCTURE_5nuc| PDB=5nuc | SCENE= }}
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'''STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT'''
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===STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT===
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==Overview==
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The structures of several variants of staphylococcal nuclease with long flexible unnatural amino acid side chains in the hydrophobic core have been determined by X-ray crystallography. The unnatural amino acids are disulfide moieties between the lone cysteine residue in V23C nuclease and methane, ethane, 1-n-propane, 1-n-butane, 1-n-pentane, and 2-hydroxyethyl thiols. We have examined changes in the core packing of these mutants. Side chains as large as the 1-n-propyl cysteine disulfide can be incorporated without perturbation of the structure. This is due, in part, to cavities present in the wild-type protein. The longest side chains are not well defined, even though they remain buried within the protein interior. These results suggest that the enthalpy-entropy balance that governs the rigidity of protein interiors favors tight packing only weakly. Additionally, the tight packing observed normally in protein interiors may reflect, in part, the limited numbers of rotamers available to the natural amino acids.
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The line below this paragraph, {{ABSTRACT_PUBMED_8762134}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8762134 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8762134}}
==About this Structure==
==About this Structure==
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[[Category: Nuclease]]
[[Category: Nuclease]]
[[Category: Unnatural amino acid]]
[[Category: Unnatural amino acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:36:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:07:29 2008''

Revision as of 11:07, 3 July 2008

Image:5nuc.png

Template:STRUCTURE 5nuc

STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT

Template:ABSTRACT PUBMED 8762134

About this Structure

5NUC is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Mobile unnatural amino acid side chains in the core of staphylococcal nuclease., Wynn R, Harkins PC, Richards FM, Fox RO, Protein Sci. 1996 Jun;5(6):1026-31. PMID:8762134

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