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7lzm

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[[Image:7lzm.gif|left|200px]]
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{{Seed}}
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[[Image:7lzm.png|left|200px]]
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{{STRUCTURE_7lzm| PDB=7lzm | SCENE= }}
{{STRUCTURE_7lzm| PDB=7lzm | SCENE= }}
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'''COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS'''
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===COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS===
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==Overview==
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Crystals of bacteriophage T4 lysozyme used for structural studies are routinely grown from concentrated phosphate solutions. It has been found that crystals in the same space group can also be grown from solutions containing 0.05 M imidazole chloride, 0.4 M sodium choride, and 30% polyethylene glycol 3500. These crystals, in addition, can also be equilibrated with a similar mother liquor in which the sodium chloride concentration is reduced to 0.025 M. The availability of these three crystal variants has permitted the structure of T4 lysozyme to be compared at low, medium, and high ionic strength. At the same time the X-ray structure of phage T4 lysozyme crystallized from phosphate solutions has been further refined against a new and improved X-ray diffraction data set. The structures of T4 lysozyme in the crystals grown with polyethylene glycol as a precipitant, regardless of the sodium chloride concentration, were very similar to the structure in crystals grown from concentrated phosphate solutions. The main differences are related to the formation of mixed disulfides between cysteine residues 54 and 97 and 2-mercaptoethanol, rather than to the differences in the salt concentration in the crystal mother liquor. Formation of the mixed disulfide at residue 54 resulted in the displacement of Arg-52 and the disruption of the salt bridge between this residue and Glu-62. Other than this change, no obvious alterations in existing salt bridges in T4 lysozyme were observed. Neither did the reduction in the ionic strength of the mother liquor result in the formation of new salt bridge interactions. These results are consistent with the ideas that a crystal structure determined at high salt concentrations is a good representation of the structure at lower ionic strengths, and that models of electrostatic interactions in proteins that are based on crystal structures determined at high salt concentrations are likely to be relevant at physiological ionic strengths.
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(as it appears on PubMed at http://www.pubmed.gov), where 2062826 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2062826}}
==About this Structure==
==About this Structure==
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[[Category: Wilson, K.]]
[[Category: Wilson, K.]]
[[Category: Zhang, X J.]]
[[Category: Zhang, X J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:46:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:41:37 2008''

Revision as of 11:41, 3 July 2008

Image:7lzm.png

Template:STRUCTURE 7lzm

COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS

Template:ABSTRACT PUBMED 2062826

About this Structure

7LZM is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Comparison of the crystal structure of bacteriophage T4 lysozyme at low, medium, and high ionic strengths., Bell JA, Wilson KP, Zhang XJ, Faber HR, Nicholson H, Matthews BW, Proteins. 1991;10(1):10-21. PMID:2062826

Page seeded by OCA on Thu Jul 3 14:41:37 2008

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