8rnt

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[[Image:8rnt.gif|left|200px]]
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{{Seed}}
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[[Image:8rnt.png|left|200px]]
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{{STRUCTURE_8rnt| PDB=8rnt | SCENE= }}
{{STRUCTURE_8rnt| PDB=8rnt | SCENE= }}
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'''STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ZINC(II) AT 1.8 ANGSTROMS RESOLUTION: A ZN2+.6H2O.CARBOXYLATE CLATHRATE'''
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===STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ZINC(II) AT 1.8 ANGSTROMS RESOLUTION: A ZN2+.6H2O.CARBOXYLATE CLATHRATE===
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==Overview==
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In order to study the inhibitory effect of Zn2+ on ribonuclease T1 [RNase T1; Itaya &amp; Inoue (1982). Biochem. J. 207, 357-362], the enzyme was cocrystallized with 2 mM Zn2+, pH 5.2, from a solution containing 55% (v/v) 2-methyl-2,4-pentanediol. The crystals are orthorhombic, P2(1)2(1)2(1), a = 48.71 (1), b = 46.51 (1), c = 41.14 (1) A, Z = 4, V = 93203 A3. The crystal structure was determined by molecular replacement and refined by restrained least-squares methods based on Fhkl for 8291 unique reflections with Fo greater than or equal to 1 sigma (Fo) in the resolution range 10 to 1.8 A and converged at a crystallographic R factor of 0.140. The Zn2+ is not bonded to the active site of RNase T1, probably because the His40 and His92 side chains are protonated. Zn2+ occupies the same site as Ca2+ in a series of crystal structures of free and nucleotide-complexed RNase T1. It is coordinated to Asp15 carboxylate and to six water molecules forming a dodecahedron of square antiprismatic form. The Zn2+...O distances are approximately 2.5 A, suggesting that Zn2+ is clathrated and not coordinated, which would require distances of 2.0 A.
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(as it appears on PubMed at http://www.pubmed.gov), where 1515106 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1515106}}
==About this Structure==
==About this Structure==
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[[Category: Granzin, J.]]
[[Category: Granzin, J.]]
[[Category: Saenger, W.]]
[[Category: Saenger, W.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:56:04 2008''

Revision as of 09:56, 11 July 2008

Image:8rnt.png

Template:STRUCTURE 8rnt

STRUCTURE OF RIBONUCLEASE T1 COMPLEXED WITH ZINC(II) AT 1.8 ANGSTROMS RESOLUTION: A ZN2+.6H2O.CARBOXYLATE CLATHRATE

Template:ABSTRACT PUBMED 1515106

About this Structure

8RNT is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Structure of ribonuclease T1 complexed with zinc(II) at 1.8 A resolution: a Zn2+.6H2O.carboxylate clathrate., Ding J, Choe HW, Granzin J, Saenger W, Acta Crystallogr B. 1992 Apr 1;48 ( Pt 2):185-91. PMID:1515106

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