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2b4z

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[[Image:2b4z.gif|left|200px]]
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{{STRUCTURE_2b4z| PDB=2b4z | SCENE= }}
{{STRUCTURE_2b4z| PDB=2b4z | SCENE= }}
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'''Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.'''
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===Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.===
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==Overview==
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Cytochrome c is one of the most studied proteins probably due to its electron-transfer properties in aerobic and anaerobic respiration. Particularly, cytochrome c from bovine heart is a small protein, M(r) 12,230 Da, globular (hydrodynamic diameter of 3.4 nm), soluble in different buffer solutions, and commercially available. Despite being a quite well-studied protein and relatively easy to manipulate from the biochemical and electrochemical viewpoint, its 3D structure has never been published. In this work, the purification, crystallization and 3D structure of one of the cytochrome c isoforms is presented to 1.5 A resolution. It is also shown how the presence of isoforms made both the purification and crystallization steps difficult. Finally, a new approach for protein electrocrystallization and design of biosensors is presented.
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(as it appears on PubMed at http://www.pubmed.gov), where 17634981 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17634981}}
==About this Structure==
==About this Structure==
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[[Category: Ferric form]]
[[Category: Ferric form]]
[[Category: Low ionic strength]]
[[Category: Low ionic strength]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:41:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:33:20 2008''

Revision as of 14:33, 27 July 2008

Image:2b4z.png

Template:STRUCTURE 2b4z

Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.

Template:ABSTRACT PUBMED 17634981

About this Structure

2B4Z is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor., Mirkin N, Jaconcic J, Stojanoff V, Moreno A, Proteins. 2008 Jan 1;70(1):83-92. PMID:17634981

Page seeded by OCA on Sun Jul 27 17:33:20 2008

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