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2zkr

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'''Structure of a mammalian Ribosomal 60S subunit within an 80S complex obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map'''
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===Structure of a mammalian Ribosomal 60S subunit within an 80S complex obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map===
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==Overview==
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In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for approximately 20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation.
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==About this Structure==
==About this Structure==
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[[Category: Protein-rna complex]]
[[Category: Protein-rna complex]]
[[Category: Ribosomal protein/rna complex]]
[[Category: Ribosomal protein/rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:52:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:00:25 2008''

Revision as of 14:00, 28 July 2008

Image:2zkr.png

Template:STRUCTURE 2zkr

Structure of a mammalian Ribosomal 60S subunit within an 80S complex obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map

Template:ABSTRACT PUBMED 18400176

About this Structure

2ZKR is a Protein complex structure of sequences from Canis lupus familiaris. Full crystallographic information is available from OCA.

Reference

Structure of the Mammalian 80S Ribosome at 8.7 A Resolution., Chandramouli P, Topf M, Menetret JF, Eswar N, Cannone JJ, Gutell RR, Sali A, Akey CW, Structure. 2008 Apr;16(4):535-48. PMID:18400176

Page seeded by OCA on Mon Jul 28 17:00:25 2008

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