3b3b

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_3b3b", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Line 1: Line 1:
-
[[Image:3b3b.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3b3b.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3b3b| PDB=3b3b | SCENE= }}
{{STRUCTURE_3b3b| PDB=3b3b | SCENE= }}
-
'''Crystal structure of E. coli Aminopeptidase N in complex with tryptophan'''
+
===Crystal structure of E. coli Aminopeptidase N in complex with tryptophan===
-
==Overview==
+
<!--
-
Aminopeptidase N from Escherichia coli is a M1 class aminopeptidase with the active-site region related to that of thermolysin. The enzyme has unusual specificity, cleaving adjacent to the large, nonpolar amino acids Phe and Tyr but also cleaving next to the polar residues Lys and Arg. To try to understand the structural basis for this pattern of hydrolysis, the structure of the enzyme was determined in complex with the amino acids l-arginine, l-lysine, l-phenylalanine, l-tryptophan, and l-tyrosine. These amino acids all bind with their backbone atoms close to the active-site zinc ion and their side chain occupying the S1 subsite. This subsite is in the form of a cylinder, about 10 A in cross-section and 12 A in length. The bottom of the cylinder includes the zinc ion and a number of polar side chains that make multiple hydrogen-bonding and other interactions with the alpha-amino group and the alpha-carboxylate of the bound amino acid. The walls of the S1 cylinder are hydrophobic and accommodate the nonpolar or largely nonpolar side chains of Phe and Tyr. The top of the cylinder is polar in character and includes bound water molecules. The epsilon-amino group of the bound lysine side chain and the guanidinium group of arginine both make multiple hydrogen bonds to this part of the S1 site. At the same time, the hydrocarbon part of the lysine and arginine side chains is accommodated within the nonpolar walls of the S1 cylinder. This combination of hydrophobic and hydrophilic binding surfaces explains the ability of ePepN to cleave Lys, Arg, Phe, and Tyr. Another favored substrate has Ala at the P1 position. The short, nonpolar side chain of this residue can clearly be bound within the hydrophobic part of the S1 cylinder, but the reason for its facile hydrolysis remains uncertain.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18416562}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18416562 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18416562}}
==About this Structure==
==About this Structure==
-
3B3B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B3B OCA].
+
3B3B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B3B OCA].
==Reference==
==Reference==
Structural Basis for the Unusual Specificity of Escherichia coli Aminopeptidase N., Addlagatta A, Gay L, Matthews BW, Biochemistry. 2008 Apr 17;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18416562 18416562]
Structural Basis for the Unusual Specificity of Escherichia coli Aminopeptidase N., Addlagatta A, Gay L, Matthews BW, Biochemistry. 2008 Apr 17;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18416562 18416562]
-
[[Category: Escherichia coli]]
+
[[Category: Escherichia coli k12]]
[[Category: Membrane alanyl aminopeptidase]]
[[Category: Membrane alanyl aminopeptidase]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 33: Line 37:
[[Category: Trptophan]]
[[Category: Trptophan]]
[[Category: Zinc]]
[[Category: Zinc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:52:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:58:34 2008''

Revision as of 18:58, 2 July 2008

Image:3b3b.png

Template:STRUCTURE 3b3b

Crystal structure of E. coli Aminopeptidase N in complex with tryptophan

Template:ABSTRACT PUBMED 18416562

About this Structure

3B3B is a Single protein structure of sequence from Escherichia coli k12. Full crystallographic information is available from OCA.

Reference

Structural Basis for the Unusual Specificity of Escherichia coli Aminopeptidase N., Addlagatta A, Gay L, Matthews BW, Biochemistry. 2008 Apr 17;. PMID:18416562

Page seeded by OCA on Wed Jul 2 21:58:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3b3b"
Personal tools