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| - | [[Image:2vmh.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2vmh| PDB=2vmh | SCENE= }} | | {{STRUCTURE_2vmh| PDB=2vmh | SCENE= }} |
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| - | '''THE STRUCTURE OF CBM51 FROM CLOSTRIDIUM PERFRINGENS GH95'''
| + | ===THE STRUCTURE OF CBM51 FROM CLOSTRIDIUM PERFRINGENS GH95=== |
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| - | ==Overview==
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| - | The genomes of myonecrotic Clostridium perfringens isolates contain genes encoding a large and fascinating array of highly modular glycoside hydrolase enzymes. Though the catalytic activities of many of these enzymes are somewhat predictable based on their amino acid sequences the functions of their abundant ancillary modules are not and these remain poorly studied. Here we present the structural and functional analysis of a new family of ancillary carbohydrate-binding module (CBM), CBM51, which was previously annotated in databases as the NPCBM domain. The high resolution crystal structures of two CBM51 examples, GH95CBM51 and GH98CBM51, from a putative family 95 alpha-fucosidase and from a family 98 blood group A/B-antigen specific endo-beta-galactosidase, respectively, showed them to have highly similar beta-sandwich folds. However, GH95CBM51 was shown by glycan microarray screening, isothermal titration calorimetry, and X-ray crystallography to bind galactose residues while the same analysis of GH98CBM51 revealed specificity for the blood group A/B-antigens through non-conserved interactions. Overall, this work identifies a new family of CBMs with many members having apparent specificity for eukaryotic glycans, in keeping with the glycan rich environment C. perfringens would experience in its host. However, a wider bioinformatic analysis of this CBM family also indicated a large number of members in non-pathogenic environmental bacteria suggesting a role in the recognition of environmental glycans. | + | The line below this paragraph, {{ABSTRACT_PUBMED_18292090}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18292090 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18292090}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Galactose]] | | [[Category: Galactose]] |
| | [[Category: Sugar-binding protein]] | | [[Category: Sugar-binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:53:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:44:58 2008'' |
Revision as of 06:45, 28 July 2008
Template:STRUCTURE 2vmh
THE STRUCTURE OF CBM51 FROM CLOSTRIDIUM PERFRINGENS GH95
Template:ABSTRACT PUBMED 18292090
About this Structure
2VMH is a Single protein structure of sequence from Clostridium perfringens. Full crystallographic information is available from OCA.
Reference
Divergent modes of glycan recognition by a new family of carbohydrate-binding modules., Gregg KJ, Finn R, Abbott DW, Boraston AB, J Biol Chem. 2008 Feb 21;. PMID:18292090
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