2jic

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{{STRUCTURE_2jic| PDB=2jic | SCENE= }}
{{STRUCTURE_2jic| PDB=2jic | SCENE= }}
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'''HIGH RESOLUTION STRUCTURE OF XYLANASE-II FROM ONE MICRON BEAM EXPERIMENT'''
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===HIGH RESOLUTION STRUCTURE OF XYLANASE-II FROM ONE MICRON BEAM EXPERIMENT===
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==Overview==
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For the first time, protein microcrystallography has been performed with a focused synchrotron-radiation beam of 1 microm using a goniometer with a sub-micrometre sphere of confusion. The crystal structure of xylanase II has been determined with a flux density of about 3 x 10(10) photons s(-1) microm(-2) at the sample. Two sets of diffraction images collected from different sized crystals were shown to comprise data of good quality, which allowed a 1.5 A resolution xylanase II structure to be obtained. The main conclusion of this experiment is that a high-resolution diffraction pattern can be obtained from 20 microm(3) crystal volume, corresponding to about 2 x 10(8) unit cells. Despite the high irradiation dose in this case, it was possible to obtain an excellent high-resolution map and it could be concluded from the individual atomic B-factor patterns that there was no evidence of significant radiation damage. The photoelectron escape from a narrow diffraction channel is a possible reason for reduced radiation damage as indicated by Monte Carlo simulations. These results open many new opportunities in scanning protein microcrystallography and make random data collection from microcrystals a real possibility, therefore enabling structures to be solved from much smaller crystals than previously anticipated as long as the crystallites are well ordered.
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(as it appears on PubMed at http://www.pubmed.gov), where 18219115 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18219115}}
==About this Structure==
==About this Structure==
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[[Category: Xylan]]
[[Category: Xylan]]
[[Category: Xylan degradation]]
[[Category: Xylan degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:20:08 2008''

Revision as of 12:20, 27 July 2008

Template:STRUCTURE 2jic

HIGH RESOLUTION STRUCTURE OF XYLANASE-II FROM ONE MICRON BEAM EXPERIMENT

Template:ABSTRACT PUBMED 18219115

About this Structure

2JIC is a Single protein structure of sequence from Trichoderma longibrachiatum. Full crystallographic information is available from OCA.

Reference

Protein crystallography with a micrometre-sized synchrotron-radiation beam., Moukhametzianov R, Burghammer M, Edwards PC, Petitdemange S, Popov D, Fransen M, McMullan G, Schertler GF, Riekel C, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):158-66. Epub 2008, Jan 16. PMID:18219115

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