2vq0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2vq0", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Line 1: Line 1:
-
[[Image:2vq0.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2vq0.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2vq0| PDB=2vq0 | SCENE= }}
{{STRUCTURE_2vq0| PDB=2vq0 | SCENE= }}
-
'''CAPSID STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN DELETION MUTANT RCP(DELTA 48 TO 59)'''
+
===CAPSID STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN DELETION MUTANT RCP(DELTA 48 TO 59)===
-
==Overview==
+
<!--
-
A unique feature of several T=3 icosahedral viruses is the presence of a structure called the beta-annulus formed by extensive hydrogen bonding between protein subunits related by icosahedral three-fold axis of symmetry. This unique structure has been suggested as a molecular switch that determines the T=3 capsid assembly. In order to examine the importance of the beta-annulus, a deletion mutant of Sesbania mosaic virus coat protein in which residues 48-59 involved in the formation of the beta-annulus were deleted retaining the rest of the residues in the amino terminal segment (rCP (Delta48-59)) was constructed. When expressed in Escherichia coli, the mutant protein assembled into virus like particles of sizes close to that of the wild type virus particles. The purified capsids were crystallized and their three dimensional structure was determined at 3.6 A resolution by X-ray crystallography. The mutant capsid structure closely resembled that of the native virus particles. However, surprisingly, the structure revealed that the assembly of the particles has proceeded without the formation of the beta-annulus. Therefore, the beta-annulus is not essential for T=3 capsid assembly as speculated earlier and may be formed as a consequence of the particle assembly. This is the first structural demonstration that the virus particle morphology with and without the beta-annulus could be closely similar.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18295296}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18295296 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18295296}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Virion]]
[[Category: Virion]]
[[Category: Virus assembly]]
[[Category: Virus assembly]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:27:16 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:54:08 2008''

Revision as of 21:54, 28 July 2008

Template:STRUCTURE 2vq0

CAPSID STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN DELETION MUTANT RCP(DELTA 48 TO 59)

Template:ABSTRACT PUBMED 18295296

About this Structure

2VQ0 is a Single protein structure of sequence from Sesbania mosaic virus. Full crystallographic information is available from OCA.

Reference

Structure of recombinant capsids formed by the beta-annulus deletion mutant - rCP (Delta48-59) of Sesbania mosaic virus., Pappachan A, Subashchandrabose C, Satheshkumar PS, Savithri HS, Murthy MR, Virology. 2008 May 25;375(1):190-6. Epub 2008 Mar 4. PMID:18295296

Page seeded by OCA on Tue Jul 29 00:54:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools