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1zmo

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[[Image:1zmo.gif|left|200px]]
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{{STRUCTURE_1zmo| PDB=1zmo | SCENE= }}
{{STRUCTURE_1zmo| PDB=1zmo | SCENE= }}
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'''Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2'''
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===Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2===
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==Overview==
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Haloalcohol dehalogenases are bacterial enzymes that cleave the carbon-halogen bond in short aliphatic vicinal haloalcohols, like 1-chloro-2,3-propanediol, some of which are recalcitrant environmental pollutants. They use a conserved Ser-Tyr-Arg catalytic triad to deprotonate the haloalcohol oxygen, which attacks the halogen-bearing carbon atom, producing an epoxide and a halide ion. Here, we present the X-ray structure of the haloalcohol dehalogenase HheA(AD2) from Arthrobacter sp. strain AD2 at 2.0-A resolution. Comparison with the previously reported structure of the 34% identical enantioselective haloalcohol dehalogenase HheC from Agrobacterium radiobacter AD1 shows that HheA(AD2) has a similar quaternary and tertiary structure but a much more open substrate-binding pocket. Docking experiments reveal that HheA(AD2) can bind both enantiomers of the haloalcohol substrate 1-p-nitrophenyl-2-chloroethanol in a productive way, which explains the low enantiopreference of HheA(AD2). Other differences are found in the halide-binding site, where the side chain amino group of Asn182 is in a position to stabilize the halogen atom or halide ion in HheA(AD2), in contrast to HheC, where a water molecule has taken over this role. These results broaden the insight into the structural determinants that govern reactivity and selectivity in the haloalcohol dehalogenase family.
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{{ABSTRACT_PUBMED_16707696}}
==About this Structure==
==About this Structure==
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Short-chain dehydrogenase/reductase family]]
[[Category: Short-chain dehydrogenase/reductase family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:36:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:45:10 2008''

Revision as of 12:45, 29 July 2008

Image:1zmo.png

Template:STRUCTURE 1zmo

Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2

Template:ABSTRACT PUBMED 16707696

About this Structure

1ZMO is a Single protein structure of sequence from Arthrobacter sp. ad2. Full crystallographic information is available from OCA.

Reference

The X-ray structure of the haloalcohol dehalogenase HheA from Arthrobacter sp. strain AD2: insight into enantioselectivity and halide binding in the haloalcohol dehalogenase family., de Jong RM, Kalk KH, Tang L, Janssen DB, Dijkstra BW, J Bacteriol. 2006 Jun;188(11):4051-6. PMID:16707696

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