1b7f
From Proteopedia
(New page: 200px<br /><applet load="1b7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b7f, resolution 2.6Å" /> '''SXL-LETHAL PROTEIN/RN...) |
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| - | [[Image:1b7f.gif|left|200px]]<br /><applet load="1b7f" size=" | + | [[Image:1b7f.gif|left|200px]]<br /><applet load="1b7f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1b7f, resolution 2.6Å" /> | caption="1b7f, resolution 2.6Å" /> | ||
'''SXL-LETHAL PROTEIN/RNA COMPLEX'''<br /> | '''SXL-LETHAL PROTEIN/RNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Sex-lethal (Sxl) protein of Drosophila melanogaster regulates | + | The Sex-lethal (Sxl) protein of Drosophila melanogaster regulates alternative splicing of the transformer (tra) messenger RNA precursor by binding to the tra polypyrimidine tract during the sex-determination process. The crystal structure has now been determined at 2.6 A resolution of the complex formed between two tandemly arranged RNA-binding domains of the Sxl protein and a 12-nucleotide, single-stranded RNA derived from the tra polypyrimidine tract. The two RNA-binding domains have their beta-sheet platforms facing each other to form a V-shaped cleft. The RNA is characteristically extended and bound in this cleft, where the UGUUUUUUU sequence is specifically recognized by the protein. This structure offers the first insight, to our knowledge, into how a protein binds specifically to a cognate RNA without any intramolecular base-pairing. |
==About this Structure== | ==About this Structure== | ||
| - | 1B7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1B7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Muto, Y.]] | [[Category: Muto, Y.]] | ||
[[Category: Nureki, O.]] | [[Category: Nureki, O.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Sakamoto, H.]] | [[Category: Sakamoto, H.]] | ||
[[Category: Shimura, Y.]] | [[Category: Shimura, Y.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:18 2008'' |
Revision as of 09:52, 21 February 2008
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SXL-LETHAL PROTEIN/RNA COMPLEX
Overview
The Sex-lethal (Sxl) protein of Drosophila melanogaster regulates alternative splicing of the transformer (tra) messenger RNA precursor by binding to the tra polypyrimidine tract during the sex-determination process. The crystal structure has now been determined at 2.6 A resolution of the complex formed between two tandemly arranged RNA-binding domains of the Sxl protein and a 12-nucleotide, single-stranded RNA derived from the tra polypyrimidine tract. The two RNA-binding domains have their beta-sheet platforms facing each other to form a V-shaped cleft. The RNA is characteristically extended and bound in this cleft, where the UGUUUUUUU sequence is specifically recognized by the protein. This structure offers the first insight, to our knowledge, into how a protein binds specifically to a cognate RNA without any intramolecular base-pairing.
About this Structure
1B7F is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein., Handa N, Nureki O, Kurimoto K, Kim I, Sakamoto H, Shimura Y, Muto Y, Yokoyama S, Nature. 1999 Apr 15;398(6728):579-85. PMID:10217141
Page seeded by OCA on Thu Feb 21 11:52:18 2008
Categories: Drosophila melanogaster | Single protein | Handa, N. | Kim, I. | Kurimoto, K. | Muto, Y. | Nureki, O. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sakamoto, H. | Shimura, Y. | Yokoyama, S. | Riken structural genomics/proteomics initiative | Rna complex | Rnp domain | Rsgi | Splicing regulation | Structural genomics
