1b8h
From Proteopedia
(New page: 200px<br /><applet load="1b8h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b8h, resolution 3.00Å" /> '''SLIDING CLAMP, DNA P...) |
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| - | [[Image:1b8h.gif|left|200px]]<br /><applet load="1b8h" size=" | + | [[Image:1b8h.gif|left|200px]]<br /><applet load="1b8h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1b8h, resolution 3.00Å" /> | caption="1b8h, resolution 3.00Å" /> | ||
'''SLIDING CLAMP, DNA POLYMERASE'''<br /> | '''SLIDING CLAMP, DNA POLYMERASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have solved the crystal structures of the bacteriophage RB69 sliding | + | We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp. |
==About this Structure== | ==About this Structure== | ||
| - | 1B8H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18]. Full crystallographic information is available from [http:// | + | 1B8H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
[[Category: accessory protein]] | [[Category: accessory protein]] | ||
[[Category: gp45]] | [[Category: gp45]] | ||
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[[Category: sliding clamp]] | [[Category: sliding clamp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:52:39 2008'' |
Revision as of 09:52, 21 February 2008
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SLIDING CLAMP, DNA POLYMERASE
Overview
We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.
About this Structure
1B8H is a Protein complex structure of sequences from Enterobacteria phage rb18. Full crystallographic information is available from OCA.
Reference
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:10535734
Page seeded by OCA on Thu Feb 21 11:52:39 2008
