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2r0f

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{{STRUCTURE_2r0f| PDB=2r0f | SCENE= }}
{{STRUCTURE_2r0f| PDB=2r0f | SCENE= }}
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'''Ligand free structure of fungal lectin CGL3'''
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===Ligand free structure of fungal lectin CGL3===
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==Overview==
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Recent advances in genome sequencing efforts have revealed an abundance of novel putative lectins. Among these, many galectin-related proteins, characterized by many conserved residues but intriguingly lacking critical amino acids, have been found in all corners of the eukaryotic superkingdom. Here we present a structural and biochemical analysis of one representative, the galectin-related lectin CGL3 found in the inky cap mushroom Coprinopsis cinerea. This protein contains all but one conserved residues known to be involved in beta-galactoside binding in galectins. A Trp residue strictly conserved among galectins is changed to an Arg in CGL3 (R81). Accordingly, the galectin-related protein is not able to bind lactose. Screening of a glycan array revealed that CGL3 displays preference for oligomers of beta1-4-linked N-acetyl-glucosamines (chitooligosaccharides) and GalNAc beta 1-4GlcNAc (LacdiNAc). Carbohydrate-binding affinity of this novel lectin was quantified using isothermal titration calorimetry, and its mode of chitooligosaccharide coordination not involving any aromatic amino acid residues was studied by X-ray crystallography. Structural information was used to alter the carbohydrate-binding specificity and substrate affinity of CGL3. The importance of residue R81 in determining the carbohydrate-binding specificity was demonstrated by replacing this Arg with a Trp residue (R81W). This single-amino-acid change led to a lectin that failed to bind chitooligosaccharides but gained lactose binding. Our results demonstrate that, similar to the legume lectin fold, the galectin fold represents a conserved structural framework upon which dramatically altered specificities can be grafted by few alterations in the binding site and that, in consequence, many metazoan galectin-related proteins may represent lectins with novel carbohydrate-binding specificities.
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==About this Structure==
==About this Structure==
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[[Category: Lectin]]
[[Category: Lectin]]
[[Category: Sugar binding protein]]
[[Category: Sugar binding protein]]
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Revision as of 02:58, 28 July 2008

Image:2r0f.png

Template:STRUCTURE 2r0f

Ligand free structure of fungal lectin CGL3

Template:ABSTRACT PUBMED 18440554

About this Structure

2R0F is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.

Reference

Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea., Walti MA, Walser PJ, Thore S, Grunler A, Bednar M, Kunzler M, Aebi M, J Mol Biol. 2008 May 23;379(1):146-59. Epub 2008 Apr 3. PMID:18440554

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