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1w9m

From Proteopedia

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{{STRUCTURE_1w9m| PDB=1w9m | SCENE= }}
{{STRUCTURE_1w9m| PDB=1w9m | SCENE= }}
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'''AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL'''
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===AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL===
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==Overview==
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The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
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{{ABSTRACT_PUBMED_18560155}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation., Aragao D, Macedo S, Mitchell EP, Romao CV, Liu MY, Frazao C, Saraiva LM, Xavier AV, LeGall J, van Dongen WM, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2003 May;8(5):540-8. Epub 2003 Feb 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12764602 12764602]
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Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution., Aragao D, Mitchell EP, Frazao CF, Carrondo MA, Lindley PF, Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):665-74. Epub 2008, May 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18560155 18560155]
[[Category: Desulfovibrio vulgaris]]
[[Category: Desulfovibrio vulgaris]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Oxidised]]
[[Category: Oxidised]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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Revision as of 17:41, 2 July 2008

Image:1w9m.png

Template:STRUCTURE 1w9m

AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL

Template:ABSTRACT PUBMED 18560155

About this Structure

1W9M is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.

Reference

Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution., Aragao D, Mitchell EP, Frazao CF, Carrondo MA, Lindley PF, Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):665-74. Epub 2008, May 14. PMID:18560155

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