3cja

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{{STRUCTURE_3cja| PDB=3cja | SCENE= }}
{{STRUCTURE_3cja| PDB=3cja | SCENE= }}
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'''Structure of Rattus norvegicus NTPDase2 in complex with calcium and AMPPNP'''
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===Structure of Rattus norvegicus NTPDase2 in complex with calcium and AMPPNP===
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==Overview==
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Cell surface-located nucleoside triphosphate diphosphohydrolases (NTPDase1, -2, -3, and -8) are oligomeric integral membrane proteins responsible for signal conversion and inactivation in extracellular nucleotide-mediated "purinergic" signaling. They catalyze the sequential hydrolysis of the signaling molecule ATP via ADP to AMP. Here we present the structure of the extracellular domain of Rattus norvegicus NTPDase2 in an active state at resolutions between 1.7 A and 2.1 A in four different forms: (i) apo form, (ii) ternary complex with the nonhydrolyzable ATP analog AMPPNP and cofactor Ca(2+), (iii) quaternary complex with Ca(2+) and bound products AMP and phosphate, and (iv) binary product complex with AMP only. Analysis of the ATP (analog) binding mode explains the importance of several residues for activity and allows suggestion of a catalytic mechanism. The carboxylate group of E165 serves as a catalytic base and activates a water molecule, which is well positioned for nucleophilic attack on the terminal phosphate. Based on analysis of the two product complex structures in which AMP adopts different conformations, a substrate binding mode for ADP hydrolysis is proposed. This allows for an understanding of how the same hydrolytic site can be engaged in ATP and ADP but not AMP hydrolysis.
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{{ABSTRACT_PUBMED_18458329}}
==About this Structure==
==About this Structure==
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[[Category: Membrane]]
[[Category: Membrane]]
[[Category: Transmembrane]]
[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:45:35 2008''

Revision as of 05:45, 29 July 2008

Image:3cja.png

Template:STRUCTURE 3cja

Structure of Rattus norvegicus NTPDase2 in complex with calcium and AMPPNP

Template:ABSTRACT PUBMED 18458329

About this Structure

3CJA is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural insight into signal conversion and inactivation by NTPDase2 in purinergic signaling., Zebisch M, Strater N, Proc Natl Acad Sci U S A. 2008 May 13;105(19):6882-7. Epub 2008 May 5. PMID:18458329

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