1bfu
From Proteopedia
(New page: 200px<br /><applet load="1bfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bfu, resolution 2.2Å" /> '''SUBTILISIN CARLSBERG ...) |
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| - | [[Image:1bfu.jpg|left|200px]]<br /><applet load="1bfu" size=" | + | [[Image:1bfu.jpg|left|200px]]<br /><applet load="1bfu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bfu, resolution 2.2Å" /> | caption="1bfu, resolution 2.2Å" /> | ||
'''SUBTILISIN CARLSBERG IN 20% DIOXANE'''<br /> | '''SUBTILISIN CARLSBERG IN 20% DIOXANE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray crystal structures of the protease subtilisin Carlsberg in 40% | + | The X-ray crystal structures of the protease subtilisin Carlsberg in 40% acetonitrile and in 20% dioxane have been determined to at least 2.3 A resolution, and their solvent binding patterns have been compared to those observed in the neat organic solvents. The structures of the protein in the two aqueous-organic mixtures are essentially the same as in pure water, acetonitrile, and dioxane. Interestingly, the enzyme-bound organic solvent molecules tend to congregate in the active site. Three of the five bound acetonitrile molecules observed in the structure of subtilisin in 40% acetonitrile are situated in the enzyme active site, as is the single enzyme-bound dioxane molecule observed in 20% dioxane (whose location is distinct from that of any bound acetonitrile molecule). Furthermore, the organic solvent molecules detected in the enzyme active site in the aqueous-organic mixtures are in the same locations as in the structures in the corresponding neat organic solvents. |
==About this Structure== | ==About this Structure== | ||
| - | 1BFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis] with CA and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] Full crystallographic information is available from [http:// | + | 1BFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Subtilisin]] | [[Category: Subtilisin]] | ||
| - | [[Category: Klibanov, A | + | [[Category: Klibanov, A M.]] |
| - | [[Category: Schmitke, J | + | [[Category: Schmitke, J L.]] |
| - | [[Category: Stern, L | + | [[Category: Stern, L J.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: DIO]] | [[Category: DIO]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:49 2008'' |
Revision as of 09:54, 21 February 2008
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SUBTILISIN CARLSBERG IN 20% DIOXANE
Overview
The X-ray crystal structures of the protease subtilisin Carlsberg in 40% acetonitrile and in 20% dioxane have been determined to at least 2.3 A resolution, and their solvent binding patterns have been compared to those observed in the neat organic solvents. The structures of the protein in the two aqueous-organic mixtures are essentially the same as in pure water, acetonitrile, and dioxane. Interestingly, the enzyme-bound organic solvent molecules tend to congregate in the active site. Three of the five bound acetonitrile molecules observed in the structure of subtilisin in 40% acetonitrile are situated in the enzyme active site, as is the single enzyme-bound dioxane molecule observed in 20% dioxane (whose location is distinct from that of any bound acetonitrile molecule). Furthermore, the organic solvent molecules detected in the enzyme active site in the aqueous-organic mixtures are in the same locations as in the structures in the corresponding neat organic solvents.
About this Structure
1BFU is a Single protein structure of sequence from Bacillus licheniformis with and as ligands. Active as Subtilisin, with EC number 3.4.21.62 Full crystallographic information is available from OCA.
Reference
Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents., Schmitke JL, Stern LJ, Klibanov AM, Biochem Biophys Res Commun. 1998 Jul 20;248(2):273-7. PMID:9675126
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