1bit
From Proteopedia
(New page: 200px<br /><applet load="1bit" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bit, resolution 1.83Å" /> '''THE CRYSTAL STRUCTUR...) |
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| - | [[Image:1bit.gif|left|200px]]<br /><applet load="1bit" size=" | + | [[Image:1bit.gif|left|200px]]<br /><applet load="1bit" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bit, resolution 1.83Å" /> | caption="1bit, resolution 1.83Å" /> | ||
'''THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM'''<br /> | '''THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined | + | Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined at 1.83 A, resolution. The crystals are orthorhombic and belong to space group P2(1)2(1)2 with lattice parameters a = 77.09, b = 82.33 and c = 31.16 A. The present structure has been compared to salmon trypsin as it appears in a previously reported crystal form (ST-IIA) with cell dimensions a = 61.95, b = 84.33 and c = 39.11 A [Smalas & Hordvik (1993). Acta Cryst. D49, 318-330]. The presence of a sulfate group involved in several hydrogen bonds to active-site residues, and the location of an additional benzamidine site in the crystal lattice, are the most striking differences between the present and the previous structure. Superposition of main-chain atoms in the two structures give an overall r.m.s. difference of 0.26 A, with the main differences located to areas with different molecular packing. The overall coordinate error is estimated to be between 0.20 and 0.25 A, by the method of Luzzati. |
==About this Structure== | ==About this Structure== | ||
| - | 1BIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CA, SO4 and BEN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1BIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BEN:'>BEN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BIT OCA]. |
==Reference== | ==Reference== | ||
Structure of anionic salmon trypsin in a second crystal form., Berglund GI, Smalas AO, Hordvik A, Willassen NP, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):725-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299802 15299802] | Structure of anionic salmon trypsin in a second crystal form., Berglund GI, Smalas AO, Hordvik A, Willassen NP, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):725-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299802 15299802] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berglund, G | + | [[Category: Berglund, G I.]] |
[[Category: BEN]] | [[Category: BEN]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:48 2008'' |
Revision as of 09:55, 21 February 2008
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THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM
Overview
Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined at 1.83 A, resolution. The crystals are orthorhombic and belong to space group P2(1)2(1)2 with lattice parameters a = 77.09, b = 82.33 and c = 31.16 A. The present structure has been compared to salmon trypsin as it appears in a previously reported crystal form (ST-IIA) with cell dimensions a = 61.95, b = 84.33 and c = 39.11 A [Smalas & Hordvik (1993). Acta Cryst. D49, 318-330]. The presence of a sulfate group involved in several hydrogen bonds to active-site residues, and the location of an additional benzamidine site in the crystal lattice, are the most striking differences between the present and the previous structure. Superposition of main-chain atoms in the two structures give an overall r.m.s. difference of 0.26 A, with the main differences located to areas with different molecular packing. The overall coordinate error is estimated to be between 0.20 and 0.25 A, by the method of Luzzati.
About this Structure
1BIT is a Single protein structure of sequence from [1] with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of anionic salmon trypsin in a second crystal form., Berglund GI, Smalas AO, Hordvik A, Willassen NP, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):725-30. PMID:15299802
Page seeded by OCA on Thu Feb 21 11:55:48 2008
Categories: Single protein | Berglund, G I. | BEN | CA | SO4 | Serine proteinase
