1ahp
From Proteopedia
(New page: 200px<br /> <applet load="1ahp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ahp, resolution 3.0Å" /> '''OLIGOSACCHARIDE SUBS...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AHP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MAL, SO4, PLP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AHP OCA]]. | + | 1AHP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MAL, SO4, PLP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phosphorylase Phosphorylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1]]. Structure known Active Site: 1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AHP OCA]]. |
==Reference== | ==Reference== | ||
Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9145112 9145112] | Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9145112 9145112] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Phosphorylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Johnson, L.N.]] | [[Category: Johnson, L.N.]] | ||
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[[Category: substrate]] | [[Category: substrate]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:34:12 2007'' |
Revision as of 10:29, 30 October 2007
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OLIGOSACCHARIDE SUBSTRATE BINDING IN ESCHERICHIA COLI MALTODEXTRIN PHSPHORYLASE
Overview
The crystal structure of E. coli maltodextrin phosphorylase, co-crystallized with an oligosaccharide has been solved at 3.0 A, resolution, providing the first structure of an oligosaccharide bound at, the catalytic site of an alpha-glucan phosphorylase. An induced fit, mechanism brings together two domains across the catalytic site tunnel. A, stacking interaction between the glucosyl residue and the aromatic group, of a tyrosine residue at a sub-site remote (8 A) from the catalytic site, provides a key element in substrate recognition; mutation of this residue, to Ala decreases the Kcat/Km by 10(4). Extrapolation of the results to, substrate binding across the site of attack by phosphorolysis indicates a, likely alteration in the glycosidic torsion angles from their preferred, values, an ... [(full description)]
About this Structure
1AHP is a [Single protein] structure of sequence from [Escherichia coli] with MAL, SO4, PLP and GOL as [ligands]. Active as [Phosphorylase], with EC number [2.4.1.1]. Structure known Active Site: 1. Full crystallographic information is available from [OCA].
Reference
Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase., O'Reilly M, Watson KA, Schinzel R, Palm D, Johnson LN, Nat Struct Biol. 1997 May;4(5):405-12. PMID:9145112
Page seeded by OCA on Tue Oct 30 12:34:12 2007
Categories: Escherichia coli | Phosphorylase | Single protein | Johnson, L.N. | Palm, D. | Reilly, M.O. | Schinzel, R. | Watson, K.A. | GOL | MAL | PLP | SO4 | Ecoli | Induced-fit | Maltodextrin | Oligosaccharide | Stacking | Substrate
