1bos
From Proteopedia
(New page: 200px<br /><applet load="1bos" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bos, resolution 2.8Å" /> '''SHIGA-LIKE TOXIN COMP...) |
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| - | [[Image:1bos.gif|left|200px]]<br /><applet load="1bos" size=" | + | [[Image:1bos.gif|left|200px]]<br /><applet load="1bos" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bos, resolution 2.8Å" /> | caption="1bos, resolution 2.8Å" /> | ||
'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''<br /> | '''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli | + | Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells. |
==About this Structure== | ==About this Structure== | ||
| - | 1BOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bacteriophage h30, bacteriophage h19b]] | [[Category: Bacteriophage h30, bacteriophage h19b]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Armstrong, G | + | [[Category: Armstrong, G D.]] |
[[Category: Boodhoo, A.]] | [[Category: Boodhoo, A.]] | ||
| - | [[Category: Brunton, J | + | [[Category: Brunton, J L.]] |
| - | [[Category: Cummings, M | + | [[Category: Cummings, M D.]] |
[[Category: Hazes, B.]] | [[Category: Hazes, B.]] | ||
[[Category: Ling, H.]] | [[Category: Ling, H.]] | ||
| - | [[Category: Read, R | + | [[Category: Read, R J.]] |
[[Category: GAL]] | [[Category: GAL]] | ||
[[Category: ob-fold]] | [[Category: ob-fold]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:28 2008'' |
Revision as of 09:57, 21 February 2008
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SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR
Overview
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
About this Structure
1BOS is a Single protein structure of sequence from Bacteriophage h30, bacteriophage h19b with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303
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