1hj6
From Proteopedia
(New page: 200px<br /> <applet load="1hj6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hj6, resolution 2.0Å" /> '''ISOCITRATE DEHYDROGE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HJ6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, IPM, NAP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ6 OCA]]. | + | 1HJ6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, IPM, NAP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Structure known Active Sites: IPM, MG and NAP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ6 OCA]]. |
==Reference== | ==Reference== | ||
Structural basis for a change in substrate specificity: crystal structure of S113E isocitrate dehydrogenase in a complex with isopropylmalate, Mg2+, and NADP., Doyle SA, Beernink PT, Koshland DE Jr, Biochemistry. 2001 Apr 10;40(14):4234-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11284679 11284679] | Structural basis for a change in substrate specificity: crystal structure of S113E isocitrate dehydrogenase in a complex with isopropylmalate, Mg2+, and NADP., Doyle SA, Beernink PT, Koshland DE Jr, Biochemistry. 2001 Apr 10;40(14):4234-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11284679 11284679] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Isocitrate dehydrogenase (NADP(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beernink, P.T.]] | [[Category: Beernink, P.T.]] | ||
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[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:34:46 2007'' |
Revision as of 10:30, 30 October 2007
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ISOCITRATE DEHYDROGENASE S113E MUTANT COMPLEXED WITH ISOPROPYLMALATE, NADP+ AND MAGNESIUM (FLASH-COOLED)
Overview
Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of, isocitrate and has negligible activity toward other (R)-malate-type, substrates. The S113E mutant of IDH significantly improves its ability to, utilize isopropylmalate as a substrate and switches the substrate, specificity (k(cat)/K(M)) from isocitrate to isopropylmalate. To, understand the structural basis for this switch in substrate specificity, we have determined the crystal structure of IDH S113E in a complex with, isopropylmalate, NADP, and Mg(2+) to 2.0 A resolution. On the basis of a, comparison with previously determined structures, we identify distinct, changes caused by the amino acid substitution and by the binding of, substrates. The S113E complex exhibits alterations in global and active, site ... [(full description)]
About this Structure
1HJ6 is a [Single protein] structure of sequence from [Escherichia coli] with MG, IPM, NAP and GOL as [ligands]. Active as [Isocitrate dehydrogenase (NADP(+))], with EC number [1.1.1.42]. Structure known Active Sites: IPM, MG and NAP. Full crystallographic information is available from [OCA].
Reference
Structural basis for a change in substrate specificity: crystal structure of S113E isocitrate dehydrogenase in a complex with isopropylmalate, Mg2+, and NADP., Doyle SA, Beernink PT, Koshland DE Jr, Biochemistry. 2001 Apr 10;40(14):4234-41. PMID:11284679
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