Sandbox 6

(Difference between revisions)

Revision as of 09:54, 3 July 2008

welcome to Strasbourg

@@ Line 1: / Line 1: @@
-<applet load='1ema' size='400' frame='true' align='right' caption='This is GFP with the chromophore bound inside the barrel' />
+welcome to Strasbourg
-== Exploring the Structure ==
-You can take a close look at the chromophore of <scene name='Sandbox_6/Gfp_scene_2/1'>GFP</scene> in the PDB entry [[1ema]]. The backbone of the entire protein is shown here on the left. The protein chain forms a <scene name='Sandbox_6/Gfp_scene_2/1'>cylindrical can</scene> (shown in blue), with one portion of the strand threading straight through the middle (shown in green). The chromophore is found right in the middle of the can, totally shielded from the surrounding environment. This shielding is essential for the fluorescence. The jostling water molecules would normally rob the chromophore of its energy once it absorbs a photon. But inside the protein, it is protected, releasing the energy instead as a slightly less energetic photon of light. The chromophore (shown in the close-up on the right) forms spontaneously from three amino acids in the protein chain: a glycine, a tyrosine and a threonine (or serine). Notice how the glycine and the threonine have formed a new bond, creating an unusual five-membered ring.
-[[Image:EriceF.gif|thumb|400px|left]]