3c70

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{{STRUCTURE_3c70| PDB=3c70 | SCENE= }}
{{STRUCTURE_3c70| PDB=3c70 | SCENE= }}
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'''HNL from Hevea brasiliensis to atomic resolution'''
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===HNL from Hevea brasiliensis to atomic resolution===
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==Overview==
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The 3D structures of complexes between the hydroxynitrile lyase from Hevea brasiliensis (Hb-HNL) and several substrate and/or inhibitor molecules, including trichloracetaldehyde, hexafluoracetone, acetone, and rhodanide, were determined by X-ray crystallography. The complex with trichloracetaldehyde showed a covalent linkage between the protein and the inhibitor, which had apparently resulted from nucleophilic attack of the catalytic Ser80-Ogamma. All other complexes showed the substrate or inhibitor molecule merely hydrogen bonded to the protein. In addition, the native crystal structure of Hb-HNL was redetermined at cryo-temperature and at room temperature, eliminating previous uncertainties concerning residual electron density within the active site, and leading to the observation of two conserved water molecules. One of them was found to be conserved in all complex structures and appears to have mainly structural significance. The other water molecule is conserved in all structures except for the complex with rhodanide; it is hydrogen bonded to the imidazole of the catalytic His235 and appears to affect the Hb-HNL catalyzed reaction. The observed 3D structural data suggest implications for the enzyme mechanism. It appears that the enzyme-catalyzed cyanohydrin formation is unlikely to proceed via a hemiacetal or hemiketal intermediate covalently attached to the enzyme, despite the observation of such an intermediate for the complex with trichloracetaldehyde. Instead, the data are consistent with a mechanism where the incoming substrate is activated by hydrogen bonding with its carbonyl oxygen to the Ser80 and Thr11 hydroxy groups. A hydrogen cyanide molecule subsequently replaces a water molecule and is deprotonated presumably by the His235 base. Deprotonation is facilitated by the proximity of the positive charge of the Lys236 side chain.
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{{ABSTRACT_PUBMED_18524775}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis., Schmidt A, Gruber K, Kratky C, Lamzin VS, J Biol Chem. 2008 Aug 1;283(31):21827-36. Epub 2008 Jun 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18524775 18524775]
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Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis., Zuegg J, Gruber K, Gugganig M, Wagner UG, Kratky C, Protein Sci. 1999 Oct;8(10):1990-2000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10548044 10548044]
Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis., Zuegg J, Gruber K, Gugganig M, Wagner UG, Kratky C, Protein Sci. 1999 Oct;8(10):1990-2000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10548044 10548044]
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Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis., Gruber K, Gugganig M, Wagner UG, Kratky C, Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10494852 10494852]
[[Category: Hevea brasiliensis]]
[[Category: Hevea brasiliensis]]
[[Category: Hydroxynitrilase]]
[[Category: Hydroxynitrilase]]
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[[Category: Protonation state]]
[[Category: Protonation state]]
[[Category: Substrate binding]]
[[Category: Substrate binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 13 13:43:52 2008''

Revision as of 10:43, 13 August 2008

Image:3c70.png

Template:STRUCTURE 3c70

HNL from Hevea brasiliensis to atomic resolution

Template:ABSTRACT PUBMED 18524775

About this Structure

3C70 is a Single protein structure of sequence from Hevea brasiliensis. Full crystallographic information is available from OCA.

Reference

Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis., Schmidt A, Gruber K, Kratky C, Lamzin VS, J Biol Chem. 2008 Aug 1;283(31):21827-36. Epub 2008 Jun 4. PMID:18524775

Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis., Zuegg J, Gruber K, Gugganig M, Wagner UG, Kratky C, Protein Sci. 1999 Oct;8(10):1990-2000. PMID:10548044

Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis., Gruber K, Gugganig M, Wagner UG, Kratky C, Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:10494852

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