3cio

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_3cio", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Line 1: Line 1:
-
[[Image:3cio.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3cio.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3cio| PDB=3cio | SCENE= }}
{{STRUCTURE_3cio| PDB=3cio | SCENE= }}
-
'''The kinase domain of Escherichia coli tyrosine kinase ETK'''
+
===The kinase domain of Escherichia coli tyrosine kinase ETK===
-
==Overview==
+
<!--
-
While protein tyrosine (Tyr) kinases (PTKs) have been extensively characterized in eukaryotes, far less is known about their emerging counterparts in prokaryotes. The inner-membrane Wzc/Etk protein belongs to the bacterial PTK family, which has an important function in regulating the polymerization and transport of virulence-determining capsular polysaccharide (CPS). The kinase uses a unique two-step activation process involving intra-phosphorylation of a Tyr residue, although the molecular mechanism remains unknown. Herein, we report the first crystal structure of a bacterial PTK, the C-terminal kinase domain of Escherichia coli Tyr kinase (Etk) at 2.5-A resolution. The fold of the Etk kinase domain differs markedly from that of eukaryotic PTKs. Based on the observed structure and supporting mass spectrometric evidence of Etk, a unique activation mechanism is proposed that involves the phosphorylated Tyr residue, Y574, at the active site and its specific interaction with a previously unidentified key Arg residue, R614, to unblock the active site. Both in vitro kinase activity and in vivo antibiotics resistance studies using structure-guided mutants further support the novel activation mechanism.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18497741}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18497741 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18497741}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Tyrosine-protein kinase]]
[[Category: Tyrosine-protein kinase]]
[[Category: Wzc]]
[[Category: Wzc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:55:14 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:09:16 2008''

Revision as of 19:09, 2 July 2008

Image:3cio.png

Template:STRUCTURE 3cio

The kinase domain of Escherichia coli tyrosine kinase ETK

Template:ABSTRACT PUBMED 18497741

About this Structure

3CIO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism., Lee DC, Zheng J, She YM, Jia Z, EMBO J. 2008 May 22;. PMID:18497741

Page seeded by OCA on Wed Jul 2 22:09:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cio"
Personal tools