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3cio

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{{STRUCTURE_3cio| PDB=3cio | SCENE= }}
{{STRUCTURE_3cio| PDB=3cio | SCENE= }}
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'''The kinase domain of Escherichia coli tyrosine kinase ETK'''
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===The kinase domain of Escherichia coli tyrosine kinase ETK===
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==Overview==
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While protein tyrosine (Tyr) kinases (PTKs) have been extensively characterized in eukaryotes, far less is known about their emerging counterparts in prokaryotes. The inner-membrane Wzc/Etk protein belongs to the bacterial PTK family, which has an important function in regulating the polymerization and transport of virulence-determining capsular polysaccharide (CPS). The kinase uses a unique two-step activation process involving intra-phosphorylation of a Tyr residue, although the molecular mechanism remains unknown. Herein, we report the first crystal structure of a bacterial PTK, the C-terminal kinase domain of Escherichia coli Tyr kinase (Etk) at 2.5-A resolution. The fold of the Etk kinase domain differs markedly from that of eukaryotic PTKs. Based on the observed structure and supporting mass spectrometric evidence of Etk, a unique activation mechanism is proposed that involves the phosphorylated Tyr residue, Y574, at the active site and its specific interaction with a previously unidentified key Arg residue, R614, to unblock the active site. Both in vitro kinase activity and in vivo antibiotics resistance studies using structure-guided mutants further support the novel activation mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 18497741 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18497741}}
==About this Structure==
==About this Structure==
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[[Category: Tyrosine-protein kinase]]
[[Category: Tyrosine-protein kinase]]
[[Category: Wzc]]
[[Category: Wzc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:55:14 2008''
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Revision as of 19:09, 2 July 2008

Image:3cio.png

Template:STRUCTURE 3cio

The kinase domain of Escherichia coli tyrosine kinase ETK

Template:ABSTRACT PUBMED 18497741

About this Structure

3CIO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism., Lee DC, Zheng J, She YM, Jia Z, EMBO J. 2008 May 22;. PMID:18497741

Page seeded by OCA on Wed Jul 2 22:09:16 2008

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