This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1bxw
From Proteopedia
(New page: 200px<br /><applet load="1bxw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bxw, resolution 2.5Å" /> '''OUTER MEMBRANE PROTEI...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1bxw.gif|left|200px]]<br /><applet load="1bxw" size=" | + | [[Image:1bxw.gif|left|200px]]<br /><applet load="1bxw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bxw, resolution 2.5Å" /> | caption="1bxw, resolution 2.5Å" /> | ||
'''OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN'''<br /> | '''OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The outer membrane protein A of Escherichia coli (OmpA) is an intensely | + | The outer membrane protein A of Escherichia coli (OmpA) is an intensely studied example in the field of membrane protein folding. We have determined the structure of the OmpA transmembrane domain consisting of residues 1-171, by X-ray diffraction analysis, to a resolution of 2.5 A. It consists of a regular, extended eight-stranded beta-barrel and appears to be constructed like an inverse micelle with large water-filled cavities, but does not form a pore. Surprisingly, the cavities seem to be highly conserved during evolution. The structure corroborates the concept that all outer membrane proteins consist of beta-barrels. The structure constitutes a beta-barrel membrane anchor that appears to be the outer membrane equivalent of the single-chain alpha-helix anchor of the inner membrane. |
==About this Structure== | ==About this Structure== | ||
| - | 1BXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with C8E as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=C8E:'>C8E</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXW OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Pautsch, A.]] | [[Category: Pautsch, A.]] | ||
| - | [[Category: Schulz, G | + | [[Category: Schulz, G E.]] |
[[Category: C8E]] | [[Category: C8E]] | ||
[[Category: outer membrane]] | [[Category: outer membrane]] | ||
[[Category: transmembrane protein]] | [[Category: transmembrane protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:00:17 2008'' |
Revision as of 10:00, 21 February 2008
|
OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN
Overview
The outer membrane protein A of Escherichia coli (OmpA) is an intensely studied example in the field of membrane protein folding. We have determined the structure of the OmpA transmembrane domain consisting of residues 1-171, by X-ray diffraction analysis, to a resolution of 2.5 A. It consists of a regular, extended eight-stranded beta-barrel and appears to be constructed like an inverse micelle with large water-filled cavities, but does not form a pore. Surprisingly, the cavities seem to be highly conserved during evolution. The structure corroborates the concept that all outer membrane proteins consist of beta-barrels. The structure constitutes a beta-barrel membrane anchor that appears to be the outer membrane equivalent of the single-chain alpha-helix anchor of the inner membrane.
About this Structure
1BXW is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the outer membrane protein A transmembrane domain., Pautsch A, Schulz GE, Nat Struct Biol. 1998 Nov;5(11):1013-7. PMID:9808047
Page seeded by OCA on Thu Feb 21 12:00:17 2008
