3d8d
From Proteopedia
(New page: '''Unreleased structure''' The entry 3d8d is ON HOLD Authors: Radzimanowski, J., Ravaud, S., Schlesinger, S., Koch, J., Beyreuther, K., Sinning, I., Wild, K. Description: Crystal struc...) |
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| - | + | [[Image:3d8d.jpg|left|200px]] | |
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| + | {{STRUCTURE_3d8d| PDB=3d8d | SCENE= }} | ||
| - | + | '''Crystal structure of the human Fe65-PTB1 domain''' | |
| - | Description: Crystal structure of the human Fe65-PTB1 domain | ||
| + | ==Overview== | ||
| + | Fe65 is a three-domain neuronal adaptor protein involved in brain development and amyloid precursor protein (APP) signalling. The phosphotyrosine-binding domain 1 (PTB1) of human Fe65 has been cloned, overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. Native crystals belong to the space group R3 and diffract to 2.6 A resolution. This crystal form suffered from high thermal B factors and pseudo-symmetry, resulting in a bisection of the c axis. Co-crystallization with a mercury compound under similar conditions induced an orthorhombic crystal form in the space group P2(1)2(1)2(1) diffracting to 2.2 A resolution. SAD phases have been computed to the diffraction limit at the wavelength of maximum absorption (L(III) edge). | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 | + | ==About this Structure== |
| + | 3D8D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D8D OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):382-5. Epub 2008 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453707 18453707] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Single protein]] | ||
| + | [[Category: Radzimanowski, J.]] | ||
| + | [[Category: Ravaud, S.]] | ||
| + | [[Category: Sinning, I.]] | ||
| + | [[Category: Wild, K.]] | ||
| + | [[Category: Alpha-beta structure]] | ||
| + | [[Category: Alternative splicing]] | ||
| + | [[Category: Phosphotyrosine binding domain]] | ||
| + | [[Category: Polymorphism]] | ||
| + | [[Category: Protein binding]] | ||
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:51:53 2008'' | ||
Revision as of 07:51, 11 June 2008
Crystal structure of the human Fe65-PTB1 domain
Overview
Fe65 is a three-domain neuronal adaptor protein involved in brain development and amyloid precursor protein (APP) signalling. The phosphotyrosine-binding domain 1 (PTB1) of human Fe65 has been cloned, overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. Native crystals belong to the space group R3 and diffract to 2.6 A resolution. This crystal form suffered from high thermal B factors and pseudo-symmetry, resulting in a bisection of the c axis. Co-crystallization with a mercury compound under similar conditions induced an orthorhombic crystal form in the space group P2(1)2(1)2(1) diffracting to 2.2 A resolution. SAD phases have been computed to the diffraction limit at the wavelength of maximum absorption (L(III) edge).
About this Structure
3D8D is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):382-5. Epub 2008 Apr 5. PMID:18453707 Page seeded by OCA on Wed Jun 11 10:51:53 2008
