1c7y
From Proteopedia
(New page: 200px<br /><applet load="1c7y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c7y, resolution 3.10Å" /> '''E.COLI RUVA-HOLLIDAY...) |
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| - | [[Image:1c7y.gif|left|200px]]<br /><applet load="1c7y" size=" | + | [[Image:1c7y.gif|left|200px]]<br /><applet load="1c7y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c7y, resolution 3.10Å" /> | caption="1c7y, resolution 3.10Å" /> | ||
'''E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX'''<br /> | '''E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In the major pathway of homologous DNA recombination in prokaryotic cells, the Holliday junction intermediate is processed through its association | + | In the major pathway of homologous DNA recombination in prokaryotic cells, the Holliday junction intermediate is processed through its association with RuvA, RuvB, and RuvC proteins. Specific binding of the RuvA tetramer to the Holliday junction is required for the RuvB motor protein to be loaded onto the junction DNA, and the RuvAB complex drives the ATP-dependent branch migration. We solved the crystal structure of the Holliday junction bound to a single Escherichia coli RuvA tetramer at 3.1-A resolution. In this complex, one side of DNA is accessible for cleavage by RuvC resolvase at the junction center. The refined junction DNA structure revealed an open concave architecture with a four-fold symmetry. Each arm, with B-form DNA, in the Holliday junction is predominantly recognized in the minor groove through hydrogen bonds with two repeated helix-hairpin-helix motifs of each RuvA subunit. The local conformation near the crossover point, where two base pairs are disrupted, suggests a possible scheme for successive base pair rearrangements, which may account for smooth Holliday junction movement without segmental unwinding. |
==About this Structure== | ==About this Structure== | ||
| - | 1C7Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1C7Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:03:20 2008'' |
Revision as of 10:03, 21 February 2008
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E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX
Overview
In the major pathway of homologous DNA recombination in prokaryotic cells, the Holliday junction intermediate is processed through its association with RuvA, RuvB, and RuvC proteins. Specific binding of the RuvA tetramer to the Holliday junction is required for the RuvB motor protein to be loaded onto the junction DNA, and the RuvAB complex drives the ATP-dependent branch migration. We solved the crystal structure of the Holliday junction bound to a single Escherichia coli RuvA tetramer at 3.1-A resolution. In this complex, one side of DNA is accessible for cleavage by RuvC resolvase at the junction center. The refined junction DNA structure revealed an open concave architecture with a four-fold symmetry. Each arm, with B-form DNA, in the Holliday junction is predominantly recognized in the minor groove through hydrogen bonds with two repeated helix-hairpin-helix motifs of each RuvA subunit. The local conformation near the crossover point, where two base pairs are disrupted, suggests a possible scheme for successive base pair rearrangements, which may account for smooth Holliday junction movement without segmental unwinding.
About this Structure
1C7Y is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer., Ariyoshi M, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8257-62. PMID:10890893
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