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2z3f

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{{STRUCTURE_2z3f| PDB=2z3f | SCENE= }}
{{STRUCTURE_2z3f| PDB=2z3f | SCENE= }}
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'''Crystal structure of spCia1/Asf1 complexed with Cac2 peptide'''
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===Crystal structure of spCia1/Asf1 complexed with Cac2 peptide===
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==Overview==
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The assembly of core histones onto eukaryotic DNA is modulated by several histone chaperone complexes, including Asf1, CAF-1, and HIRA. Asf1 is a unique histone chaperone that participates in both the replication-dependent and replication-independent pathways. Here we report the crystal structures of the apo-form of fission yeast Asf1/Cia1 (SpAsf1N; residues 1-161) as well as its complexes with the B-domain of the fission yeast HIRA orthologue Hip1 (Hip1B) and the C-terminal region of the Cac2 subunit of CAF-1 (Cac2C). The mode of the fission yeast Asf1N-Hip1B recognition is similar to that of the human Asf1-HIRA recognition, suggesting that Asf1N recognition of Hip1B/HIRA is conserved from yeast to mammals. Interestingly, Hip1B and Cac2C show remarkably similar interaction modes with Asf1. The binding between Asf1N and Hip1B was almost completely abolished by the D37A and L60A/V62A mutations in Asf1N, indicating the critical role of salt bridge and van der Waals contacts in the complex formation. Consistently, both of the aforementioned Asf1 mutations also drastically reduced the binding to Cac2C. These results provide a structural basis for a mutually exclusive Asf1-binding model of CAF-1 and HIRA/Hip1, in which Asf1 and CAF-1 assemble histones H3/H4 (H3.1/H4 in vertebrates) in a replication-dependent pathway, whereas Asf1 and HIRA/Hip1 assemble histones H3/H4 (H3.3/H4 in vertebrates) in a replication-independent pathway.
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{{ABSTRACT_PUBMED_18334479}}
==About this Structure==
==About this Structure==
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Wd repeat]]
[[Category: Wd repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:06:32 2008''

Revision as of 20:06, 27 July 2008

Image:2z3f.png

Template:STRUCTURE 2z3f

Crystal structure of spCia1/Asf1 complexed with Cac2 peptide

Template:ABSTRACT PUBMED 18334479

About this Structure

2Z3F is a Protein complex structure of sequences from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

Reference

Crystal Structures of Fission Yeast Histone Chaperone Asf1 Complexed with the Hip1 B-domain or the Cac2 C Terminus., Malay AD, Umehara T, Matsubara-Malay K, Padmanabhan B, Yokoyama S, J Biol Chem. 2008 May 16;283(20):14022-31. Epub 2008 Mar 11. PMID:18334479

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