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3be0

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{{STRUCTURE_3be0| PDB=3be0 | SCENE= }}
{{STRUCTURE_3be0| PDB=3be0 | SCENE= }}
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'''The Role of Asn 242 in P450cin'''
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===The Role of Asn 242 in P450cin===
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==Overview==
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Cytochrome P450cin (CYP176A1) is a bacterial P450 isolated from Citrobacter braakii that catalyzes the hydroxylation of cineole to (S)-6beta-hydroxycineole. This initiates the biodegradation of cineole, enabling C. braakii to live on cineole as its sole source of carbon and energy. P450cin lacks the almost universally conserved threonine residue believed to be involved in dioxygen activation and instead contains an asparagine at this position (Asn-242). To investigate the role of Asn-242 in P450cin catalysis, it was converted to alanine, and the resultant mutant was characterized. The characteristic CO-bound spectrum and spectrally determined K(D) for substrate binding were unchanged in the mutant. The x-ray crystal structures of the substrate-free and -bound N242A mutant were determined and show that the only significant change is in a reorientation of the substrate such that (R)-6alpha-hydroxycineole should be a major product. Molecular dynamics simulations of both wild type and mutant are consistent with the change in regio- and stereoselectivity predicted from the crystal structure. The mutation has only a modest effect on enzyme activity and on the diversion of the NADPH-reducing equivalent toward unproductive peroxide formation. Product profile analysis shows that (R)-6alpha-hydroxycineole is the main product, which is consistent with the crystal structure. These results demonstrate that Asn-242 is not a functional replacement for the conserved threonine in other P450s but, rather, is critical in controlling regioselective substrate oxidation.
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{{ABSTRACT_PUBMED_18270198}}
==About this Structure==
==About this Structure==
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[[Category: Protein substrate complex]]
[[Category: Protein substrate complex]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:56:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:39:22 2008''

Revision as of 04:39, 29 July 2008

Image:3be0.png

Template:STRUCTURE 3be0

The Role of Asn 242 in P450cin

Template:ABSTRACT PUBMED 18270198

About this Structure

3BE0 is a Single protein structure of sequence from Citrobacter braakii. Full crystallographic information is available from OCA.

Reference

The critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin., Meharenna YT, Slessor KE, Cavaignac SM, Poulos TL, De Voss JJ, J Biol Chem. 2008 Apr 18;283(16):10804-12. Epub 2008 Feb 12. PMID:18270198

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