1c9i
From Proteopedia
(New page: 200px<br /><applet load="1c9i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c9i, resolution 2.90Å" /> '''PEPTIDE-IN-GROOVE IN...) |
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| - | [[Image:1c9i.jpg|left|200px]]<br /><applet load="1c9i" size=" | + | [[Image:1c9i.jpg|left|200px]]<br /><applet load="1c9i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1c9i, resolution 2.90Å" /> | caption="1c9i, resolution 2.90Å" /> | ||
'''PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN'''<br /> | '''PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The "WD40" domain is a widespread recognition module for linking partner | + | The "WD40" domain is a widespread recognition module for linking partner proteins in intracellular networks of signaling and sorting. The clathrin amino-terminal domain, which directs incorporation of cargo into coated pits, is a beta-propeller closely related in structure to WD40 modules. The crystallographically determined structures of complexes of the clathrin-terminal domain with peptides derived from two different cargo adaptors, beta-arrestin 2 and the beta-subunit of the AP-3 complex, reveal strikingly similar peptide-in-groove interactions. The two peptides in our structures contain related, five-residue motifs, which form the core of their contact with clathrin. A number of other proteins involved in endocytosis have similar "clathrin-box" motifs, and it therefore is likely that they all bind the terminal domain in the same way. We propose that a peptide-in-groove interaction is an important general mode by which beta-propellers recognize specific target proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1C9I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 1C9I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Haar, E | + | [[Category: Haar, E ter.]] |
[[Category: Harrison, S. C.]] | [[Category: Harrison, S. C.]] | ||
[[Category: Kirchhausen, T.]] | [[Category: Kirchhausen, T.]] | ||
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[[Category: helical hairpin]] | [[Category: helical hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:03:53 2008'' |
Revision as of 10:03, 21 February 2008
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PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN
Overview
The "WD40" domain is a widespread recognition module for linking partner proteins in intracellular networks of signaling and sorting. The clathrin amino-terminal domain, which directs incorporation of cargo into coated pits, is a beta-propeller closely related in structure to WD40 modules. The crystallographically determined structures of complexes of the clathrin-terminal domain with peptides derived from two different cargo adaptors, beta-arrestin 2 and the beta-subunit of the AP-3 complex, reveal strikingly similar peptide-in-groove interactions. The two peptides in our structures contain related, five-residue motifs, which form the core of their contact with clathrin. A number of other proteins involved in endocytosis have similar "clathrin-box" motifs, and it therefore is likely that they all bind the terminal domain in the same way. We propose that a peptide-in-groove interaction is an important general mode by which beta-propellers recognize specific target proteins.
About this Structure
1C9I is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Peptide-in-groove interactions link target proteins to the beta-propeller of clathrin., ter Haar E, Harrison SC, Kirchhausen T, Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1096-100. PMID:10655490
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