2q9f

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{{STRUCTURE_2q9f| PDB=2q9f | SCENE= }}
{{STRUCTURE_2q9f| PDB=2q9f | SCENE= }}
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'''Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate'''
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===Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate===
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==Overview==
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Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogue cholesterol-3-sulfate (CH-3S) is reported. The 2.6 angstroms structure of CYP46A1-CH-3S was solved using both anion and cation heavy-atom salts. In addition to the native anomalous signal from the haem iron, an NaI anion halide salt derivative and a complementary CsCl alkali-metal cation salt derivative were used. The general implications of the use of halide and alkali-metal quick soaks are discussed. The importance of using isoionic strength buffers, the titration of heavy-atom salts into different ionic species and the role of concentration are considered. It was observed that cation/anion-binding sites will occasionally overlap, which could negatively impact upon mixed RbBr soaks used for multiple anomalous scatterer MAD (MMAD). The use of complementary cation and anion heavy-atom salt derivatives is a convenient and powerful tool for MIR(AS) structure solution.
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(as it appears on PubMed at http://www.pubmed.gov), where 18621681 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18621681}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain., Mast N, White MA, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9546-51. Epub 2008 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18621681 18621681]
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Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453684 18453684]
Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453684 18453684]
[[Category: Cholesterol 24-hydroxylase]]
[[Category: Cholesterol 24-hydroxylase]]
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[[Category: P450]]
[[Category: P450]]
[[Category: P450 46a1]]
[[Category: P450 46a1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:03:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 6 09:49:09 2008''

Revision as of 06:49, 6 August 2008

Image:2q9f.png

Template:STRUCTURE 2q9f

Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate

Template:ABSTRACT PUBMED 18621681

About this Structure

2Q9F is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain., Mast N, White MA, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9546-51. Epub 2008 Jul 9. PMID:18621681

Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1., White MA, Mast N, Bjorkhem I, Johnson EF, Stout CD, Pikuleva IA, Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):487-95. Epub 2008, Apr 19. PMID:18453684

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