2rif

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{{STRUCTURE_2rif| PDB=2rif | SCENE= }}
{{STRUCTURE_2rif| PDB=2rif | SCENE= }}
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'''CBS domain protein PAE2072 from Pyrobaculum aerophilum complexed with AMP'''
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===CBS domain protein PAE2072 from Pyrobaculum aerophilum complexed with AMP===
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==Overview==
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Cystathionine beta-synthase domains are found in a myriad of proteins from organisms across the tree of life and have been hypothesized to function as regulatory modules that sense the energy charge of cells. Here we characterize the structure and stability of PAE2072, a dimeric tandem cystathionine beta-synthase domain protein from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. Crystal structures of the protein in unliganded and AMP-bound forms, determined at resolutions of 2.10 and 2.35 A, respectively, reveal remarkable conservation of key functional features seen in the gamma subunit of the eukaryotic AMP-activated protein kinase. The structures also confirm the presence of a suspected intermolecular disulfide bond between the two subunits that is shown to stabilize the protein. Our AMP-bound structure represents a first step in investigating the function of a large class of uncharacterized prokaryotic proteins. In addition, this work extends previous studies that have suggested that, in certain thermophilic microbes, disulfide bonds play a key role in stabilizing intracellular proteins and protein-protein complexes.
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{{ABSTRACT_PUBMED_18513746}}
==About this Structure==
==About this Structure==
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[[Category: Ligand-binding protein]]
[[Category: Ligand-binding protein]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:04:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:41:40 2008''

Revision as of 08:41, 29 July 2008

Image:2rif.png

Template:STRUCTURE 2rif

CBS domain protein PAE2072 from Pyrobaculum aerophilum complexed with AMP

Template:ABSTRACT PUBMED 18513746

About this Structure

2RIF is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.

Reference

Structures and functional implications of an AMP-binding cystathionine beta-synthase domain protein from a hyperthermophilic archaeon., King NP, Lee TM, Sawaya MR, Cascio D, Yeates TO, J Mol Biol. 2008 Jun 27;380(1):181-92. Epub 2008 May 7. PMID:18513746

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