1m5k

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[[Image:1m5k.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1m5k| PDB=1m5k | SCENE= }}
{{STRUCTURE_1m5k| PDB=1m5k | SCENE= }}
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'''Crystal structure of a hairpin ribozyme in the catalytically-active conformation'''
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===Crystal structure of a hairpin ribozyme in the catalytically-active conformation===
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==Overview==
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The hairpin ribozyme catalyses sequence-specific cleavage of RNA. The active site of this natural RNA results from the docking of two irregular helices: stems A and B. One strand of stem A harbours the scissile bond. The 2.4 A resolution structure of a hairpin ribozyme-inhibitor complex reveals that the ribozyme aligns the 2'-OH nucleophile and the 5'-oxo leaving group by twisting apart the nucleotides that flank the scissile phosphate. The base of the nucleotide preceding the cleavage site is stacked within stem A; the next nucleotide, a conserved guanine, is extruded from stem A and accommodated by a highly complementary pocket in the minor groove of stem B. Metal ions are absent from the active site. The bases of four conserved purines are positioned potentially to serve as acid-base catalysts. This is the first structure determination of a fully assembled ribozyme active site that catalyses a phosphodiester cleavage without recourse to metal ions.
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(as it appears on PubMed at http://www.pubmed.gov), where 11298439 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11298439}}
==About this Structure==
==About this Structure==
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[[Category: Translation/rna complex]]
[[Category: Translation/rna complex]]
[[Category: U1a rna binding protein docked conformation]]
[[Category: U1a rna binding protein docked conformation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:13:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 20:32:20 2008''

Revision as of 17:32, 2 July 2008

Image:1m5k.png

Template:STRUCTURE 1m5k

Crystal structure of a hairpin ribozyme in the catalytically-active conformation

Template:ABSTRACT PUBMED 11298439

About this Structure

1M5K is a Protein complex structure of sequences from Homo sapiens. This structure supersedes the now removed PDB entry 1hp6. Full crystallographic information is available from OCA.

Reference

Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis., Rupert PB, Ferre-D'Amare AR, Nature. 2001 Apr 12;410(6830):780-6. PMID:11298439

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