2dyh

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{{STRUCTURE_2dyh| PDB=2dyh | SCENE= }}
{{STRUCTURE_2dyh| PDB=2dyh | SCENE= }}
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'''Crystal structure of the Keap1 protein in complexed with the N-terminal region of the Nrf2 transcription factor'''
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===Crystal structure of the Keap1 protein in complexed with the N-terminal region of the Nrf2 transcription factor===
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==Overview==
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Nrf2 is the regulator of the oxidative/electrophilic stress response. Its turnover is maintained by Keap1-mediated proteasomal degradation via a two-site substrate recognition mechanism in which two Nrf2-Keap1 binding sites form a hinge and latch. The E3 ligase adaptor Keap1 recognizes Nrf2 through its conserved ETGE and DLG motifs. In this study, we examined how the ETGE and DLG motifs bind to Keap1 in a very similar fashion but with different binding affinities by comparing the crystal complex of a Keap1-DC domain-DLG peptide with that of a Keap1-DC domain-ETGE peptide. We found that these two motifs interact with the same basic surface of either Keap1-DC domain of the Keap1 homodimer. The DLG motif works to correctly position the lysines within the Nrf2 Neh2 domain for efficient ubiquitination. Together with the results from calorimetric and functional studies, we conclude that different electrostatic potentials primarily define the ETGE and DLG motifs as a hinge and latch that senses the oxidative/electrophilic stress.
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(as it appears on PubMed at http://www.pubmed.gov), where 17785452 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17785452}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:14:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:20:15 2008''

Revision as of 09:20, 29 July 2008

Image:2dyh.png

Template:STRUCTURE 2dyh

Crystal structure of the Keap1 protein in complexed with the N-terminal region of the Nrf2 transcription factor

Template:ABSTRACT PUBMED 17785452

About this Structure

2DYH is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Different electrostatic potentials define ETGE and DLG motifs as hinge and latch in oxidative stress response., Tong KI, Padmanabhan B, Kobayashi A, Shang C, Hirotsu Y, Yokoyama S, Yamamoto M, Mol Cell Biol. 2007 Nov;27(21):7511-21. Epub 2007 Sep 4. PMID:17785452

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