2h60

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{{STRUCTURE_2h60| PDB=2h60 | SCENE= }}
{{STRUCTURE_2h60| PDB=2h60 | SCENE= }}
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'''Solution Structure of Human Brg1 Bromodomain'''
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===Solution Structure of Human Brg1 Bromodomain===
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==Overview==
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Human brahma-related gene 1 (Brg1) is a core protein in human SWI/SNF chromatin-remodeling complex which regulates gene expression. Brg1 contains a bromodomain that has been shown to anchor the entire complex to promoter nucleosomes by interacting with histones that are acetylated at specific lysine residues. The Brg1 bromodomain belongs to an important subclass of the bromodomain family for which no structural information is known. Here we report the solution structure of the Brg1 bromodomain determined by NMR. The Brg1 bromodomain conserves the left-handed, four-helix bundle topology found in other bromodomain structures. However, the alphaZ helix of Brg1 bromodomain is about 4 residues shorter relative to previously published bromodomain structures. Using NMR perturbation studies, we demonstrate the Brg1 bromodomain binds acetyllysine in the context of histone tails, with no comparable affinity for unacetylated peptides. The estimated dissociation constants (KD) for acetylated histone peptides H4-AcK8 and H4-AcK12 are 4.0 and 3.6 mM, respectively. In this study the dominant substrate was H3-AcK14 (KD approximately 1.2 mM). Mutagenesis analysis reveals several residues important for the binding specificity. Using molecular dynamics simulations, we present a model of the Brg1 bromodomain in complex with H3-AcK14 and discuss the potential interactions which provide the selectivity of the Brg1 bromodomain for histone H3-AcK14.
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==About this Structure==
==About this Structure==
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2H60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H60 OCA].
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2H60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H60 OCA].
==Reference==
==Reference==
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[[Category: Alfa helix]]
[[Category: Alfa helix]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:15:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:25:35 2008''

Revision as of 12:25, 27 July 2008

Image:2h60.png

Template:STRUCTURE 2h60

Solution Structure of Human Brg1 Bromodomain

Template:ABSTRACT PUBMED 17274598

About this Structure

2H60 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of human Brg1 bromodomain and its specific binding to acetylated histone tails., Shen W, Xu C, Huang W, Zhang J, Carlson JE, Tu X, Wu J, Shi Y, Biochemistry. 2007 Feb 27;46(8):2100-10. Epub 2007 Feb 3. PMID:17274598

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