2inv

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{{STRUCTURE_2inv| PDB=2inv | SCENE= }}
{{STRUCTURE_2inv| PDB=2inv | SCENE= }}
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'''Crystal structure of inulin fructotransferase in the presence of di-fructose'''
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===Crystal structure of inulin fructotransferase in the presence of di-fructose===
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==Overview==
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Inulin fructotransferase (IFTase), a member of glycoside hydrolase family 91, catalyzes depolymerization of beta-2,1-fructans inulin by successively removing the terminal difructosaccharide units as cyclic anhydrides via intramolecular fructosyl transfer. The crystal structures of IFTase and its substrate-bound complex reveal that IFTase is a trimeric enzyme, and each monomer folds into a right-handed parallel beta-helix. Despite variation in the number and conformation of its beta-strands, the IFTase beta-helix has a structure that is largely reminiscent of other beta-helix structures but is unprecedented in that trimerization is a prerequisite for catalytic activity, and the active site is located at the monomer-monomer interface. Results from crystallographic studies and site-directed mutagenesis provide a structural basis for the exolytic-type activity of IFTase and a functional resemblance to inverting-type glycosyltransferases.
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(as it appears on PubMed at http://www.pubmed.gov), where 17192265 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17192265}}
==About this Structure==
==About this Structure==
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[[Category: Protein-carbohydrate complex]]
[[Category: Protein-carbohydrate complex]]
[[Category: Right-handed parallel beta-helix]]
[[Category: Right-handed parallel beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:15:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:15:36 2008''

Revision as of 02:15, 29 July 2008

Image:2inv.png

Template:STRUCTURE 2inv

Crystal structure of inulin fructotransferase in the presence of di-fructose

Template:ABSTRACT PUBMED 17192265

About this Structure

2INV is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Structural and functional insights into intramolecular fructosyl transfer by inulin fructotransferase., Jung WS, Hong CK, Lee S, Kim CS, Kim SJ, Kim SI, Rhee S, J Biol Chem. 2007 Mar 16;282(11):8414-23. Epub 2006 Dec 27. PMID:17192265

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