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3cke

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{{STRUCTURE_3cke| PDB=3cke | SCENE= }}
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'''Crystal structure of aristolochene synthase in complex with 12,13-difluorofarnesyl diphosphate'''
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===Crystal structure of aristolochene synthase in complex with 12,13-difluorofarnesyl diphosphate===
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==Overview==
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The universal sesquiterpene precursor, farnesyl diphosphate (FPP), is cyclized in an Mg(2+)-dependent reaction catalyzed by the tetrameric aristolochene synthase from Aspergillus terreus to form the bicyclic hydrocarbon aristolochene and a pyrophosphate anion (PP(i)) coproduct. The 2.1-A resolution crystal structure determined from crystals soaked with FPP reveals the binding of intact FPP to monomers A-C, and the binding of PP(i) and Mg(2+)(B) to monomer D. The 1.89-A resolution structure of the complex with 2-fluorofarnesyl diphosphate (2F-FPP) reveals 2F-FPP binding to all subunits of the tetramer, with Mg(2+)(B)accompanying the binding of this analogue only in monomer D. All monomers adopt open activesite conformations in these complexes, but slight structural changes in monomers C and D of each complex reflect the very initial stages of a conformational transition to the closed state. Finally, the 2.4-A resolution structure of the complex with 12,13-difluorofarnesyl diphosphate (DF-FPP) reveals the binding of intact DF-FPP to monomers A-C in the open conformation and the binding of PP(i), Mg(2+)(B), and Mg(2+)(C) to monomer D in a predominantly closed conformation. Taken together, these structures provide 12 independent "snapshots" of substrate or product complexes that suggest a possible sequence for metal ion binding and conformational changes required for catalysis.
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{{ABSTRACT_PUBMED_18385128}}
==About this Structure==
==About this Structure==
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[[Category: Metal ion binding]]
[[Category: Metal ion binding]]
[[Category: Substrate binding]]
[[Category: Substrate binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:31:28 2008''

Revision as of 14:31, 27 July 2008

Image:3cke.png

Template:STRUCTURE 3cke

Crystal structure of aristolochene synthase in complex with 12,13-difluorofarnesyl diphosphate

Template:ABSTRACT PUBMED 18385128

About this Structure

3CKE is a Single protein structure of sequence from Aspergillus terreus. Full crystallographic information is available from OCA.

Reference

X-ray Crystallographic Studies of Substrate Binding to Aristolochene Synthase Suggest a Metal Ion Binding Sequence for Catalysis., Shishova EY, Yu F, Miller DJ, Faraldos JA, Zhao Y, Coates RM, Allemann RK, Cane DE, Christianson DW, J Biol Chem. 2008 May 30;283(22):15431-9. Epub 2008 Apr 2. PMID:18385128

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