1cej
From Proteopedia
(New page: 200px<br /><applet load="1cej" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cej" /> '''SOLUTION STRUCTURE OF AN EGF MODULE PAIR FRO...) |
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'''SOLUTION STRUCTURE OF AN EGF MODULE PAIR FROM THE PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1'''<br /> | '''SOLUTION STRUCTURE OF AN EGF MODULE PAIR FROM THE PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of the 96-residue C-terminal fragment of the | + | The solution structure of the 96-residue C-terminal fragment of the merozoite surface protein 1 (MSP-1) from Plasmodium falciparum has been determined using nuclear magnetic resonance (NMR) spectroscopic measurements on uniformly13C/15N-labelled protein, efficiently expressed in the methylotrophic yeast Komagataella (Pichia) pastoris. The structure has two domains with epidermal growth factor (EGF)-like folds with a novel domain interface for the EGF domain pair interactions, formed from a cluster of hydrophobic residues. This gives the protein a U-shaped overall structure with the N-terminal proteolytic processing site close to the C-terminal glycosyl phosphatidyl inositol (GPI) membrane anchor site, which is consistent with the involvement of a membrane-bound proteinase in the processing of MSP-1 during erythrocyte invasion. This structure, which is the first protozoan EGF example to be determined, contrasts with the elongated structures seen for EGF-module pairs having shared Ca2+-ligation sites at their interface, as found, for example, in fibrillin-1. Recognition surfaces for antibodies that inhibit processing and invasion, and antibodies that block the binding of these inhibitory antibodies, have been mapped on the three-dimensional structure by considering specific MSP-1 mutants. |
==About this Structure== | ==About this Structure== | ||
| - | 1CEJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http:// | + | 1CEJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Birdsall, B.]] | [[Category: Birdsall, B.]] | ||
| - | [[Category: Burghaus, P | + | [[Category: Burghaus, P A.]] |
[[Category: Feeney, J.]] | [[Category: Feeney, J.]] | ||
| - | [[Category: Frenkiel, T | + | [[Category: Frenkiel, T A.]] |
| - | [[Category: Gradwell, M | + | [[Category: Gradwell, M G.]] |
| - | [[Category: Holder, A | + | [[Category: Holder, A A.]] |
| - | [[Category: Morgan, W | + | [[Category: Morgan, W D.]] |
| - | [[Category: Syed, S | + | [[Category: Syed, S E.H.]] |
[[Category: Uthaipibull, C.]] | [[Category: Uthaipibull, C.]] | ||
[[Category: egf-like domain]] | [[Category: egf-like domain]] | ||
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[[Category: surface antigen]] | [[Category: surface antigen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:15 2008'' |
Revision as of 10:05, 21 February 2008
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SOLUTION STRUCTURE OF AN EGF MODULE PAIR FROM THE PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1
Overview
The solution structure of the 96-residue C-terminal fragment of the merozoite surface protein 1 (MSP-1) from Plasmodium falciparum has been determined using nuclear magnetic resonance (NMR) spectroscopic measurements on uniformly13C/15N-labelled protein, efficiently expressed in the methylotrophic yeast Komagataella (Pichia) pastoris. The structure has two domains with epidermal growth factor (EGF)-like folds with a novel domain interface for the EGF domain pair interactions, formed from a cluster of hydrophobic residues. This gives the protein a U-shaped overall structure with the N-terminal proteolytic processing site close to the C-terminal glycosyl phosphatidyl inositol (GPI) membrane anchor site, which is consistent with the involvement of a membrane-bound proteinase in the processing of MSP-1 during erythrocyte invasion. This structure, which is the first protozoan EGF example to be determined, contrasts with the elongated structures seen for EGF-module pairs having shared Ca2+-ligation sites at their interface, as found, for example, in fibrillin-1. Recognition surfaces for antibodies that inhibit processing and invasion, and antibodies that block the binding of these inhibitory antibodies, have been mapped on the three-dimensional structure by considering specific MSP-1 mutants.
About this Structure
1CEJ is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1., Morgan WD, Birdsall B, Frenkiel TA, Gradwell MG, Burghaus PA, Syed SE, Uthaipibull C, Holder AA, Feeney J, J Mol Biol. 1999 May 28;289(1):113-22. PMID:10339410
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