1cf2
From Proteopedia
(New page: 200px<br /><applet load="1cf2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cf2, resolution 2.100Å" /> '''THREE-DIMENSIONAL S...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1cf2.jpg|left|200px]]<br /><applet load="1cf2" size=" | + | [[Image:1cf2.jpg|left|200px]]<br /><applet load="1cf2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cf2, resolution 2.100Å" /> | caption="1cf2, resolution 2.100Å" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The homotetrameric holo-D-glyceraldehyde-3-phosphate dehydrogenase from | + | The homotetrameric holo-D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus has been crystallized in the presence of NADP+ using the hanging-drop vapour-diffusion method. Crystals grew from a solution containing 2-methyl-2,4-pentanediol and magnesium acetate. A native data set has been collected to 2.1 A using synchrotron radiation and cryocooling. Diffraction data have been processed in the orthorhombic system (space group P21212) with unit-cell dimensions a = 136.7, b = 153.3, c = 74.9 A and one tetramer per asymmetric unit. |
==About this Structure== | ==About this Structure== | ||
| - | 1CF2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermus_fervidus Methanothermus fervidus] with SO4 and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http:// | + | 1CF2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermus_fervidus Methanothermus fervidus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CF2 OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 17: | ||
[[Category: Branlant, G.]] | [[Category: Branlant, G.]] | ||
[[Category: Charron, C.]] | [[Category: Charron, C.]] | ||
| - | [[Category: Isuppov, M | + | [[Category: Isuppov, M N.]] |
| - | [[Category: Littlechild, J | + | [[Category: Littlechild, J A.]] |
[[Category: Talfournier, F.]] | [[Category: Talfournier, F.]] | ||
[[Category: Vitoux, B.]] | [[Category: Vitoux, B.]] | ||
| Line 25: | Line 25: | ||
[[Category: oxydoreductase]] | [[Category: oxydoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:25 2008'' |
Revision as of 10:05, 21 February 2008
|
THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS
Overview
The homotetrameric holo-D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus has been crystallized in the presence of NADP+ using the hanging-drop vapour-diffusion method. Crystals grew from a solution containing 2-methyl-2,4-pentanediol and magnesium acetate. A native data set has been collected to 2.1 A using synchrotron radiation and cryocooling. Diffraction data have been processed in the orthorhombic system (space group P21212) with unit-cell dimensions a = 136.7, b = 153.3, c = 74.9 A and one tetramer per asymmetric unit.
About this Structure
1CF2 is a Single protein structure of sequence from Methanothermus fervidus with and as ligands. Active as Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), with EC number 1.2.1.12 Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus., Charron C, Talfournier F, Isupov MN, Branlant G, Littlechild JA, Vitoux B, Aubry A, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1353-5. PMID:10393306
Page seeded by OCA on Thu Feb 21 12:05:25 2008
