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1cgt

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(New page: 200px<br /><applet load="1cgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgt, resolution 2.0&Aring;" /> '''STRUCTURE OF CYCLODEX...)
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[[Image:1cgt.gif|left|200px]]<br /><applet load="1cgt" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1cgt, resolution 2.0&Aring;" />
caption="1cgt, resolution 2.0&Aring;" />
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION'''<br />
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION'''<br />
==Overview==
==Overview==
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The previously reported structural model of cyclodextrin, glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been, improved. For this purpose the known sequence was built into an electron, density map established by multiple isomorphous replacement and subsequent, solvent-flattening at 2.5 A resolution. The resulting model was refined at, 2.0 A resolution using a simulated annealing refinement method. Based on, 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a, final R-factor of 17.6% was obtained with a model obeying standard, geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles., The final model consists of all 684 amino acid residues, two calcium ions, and 588 solvent molecules.
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The previously reported structural model of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been improved. For this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 A resolution. The resulting model was refined at 2.0 A resolution using a simulated annealing refinement method. Based on 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a final R-factor of 17.6% was obtained with a model obeying standard geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles. The final model consists of all 684 amino acid residues, two calcium ions and 588 solvent molecules.
==About this Structure==
==About this Structure==
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1CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CGT OCA].
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1CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGT OCA].
==Reference==
==Reference==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Klein, C.]]
[[Category: Klein, C.]]
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[[Category: Schulz, G.E.]]
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[[Category: Schulz, G E.]]
[[Category: CA]]
[[Category: CA]]
[[Category: glycosyltransferase]]
[[Category: glycosyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:26:14 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:57 2008''

Revision as of 10:05, 21 February 2008

Image:1cgt.gif

1cgt, resolution 2.0Å

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STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION

Overview

The previously reported structural model of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been improved. For this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 A resolution. The resulting model was refined at 2.0 A resolution using a simulated annealing refinement method. Based on 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a final R-factor of 17.6% was obtained with a model obeying standard geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles. The final model consists of all 684 amino acid residues, two calcium ions and 588 solvent molecules.

About this Structure

1CGT is a Single protein structure of sequence from Bacillus circulans with as ligand. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.

Reference

Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution., Klein C, Schulz GE, J Mol Biol. 1991 Feb 20;217(4):737-50. PMID:1826034

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