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1cmg

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(New page: 200px<br /><applet load="1cmg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cmg" /> '''NMR SOLUTION STRUCTURE OF CALCIUM-LOADED CAL...)
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[[Image:1cmg.gif|left|200px]]<br /><applet load="1cmg" size="350" color="white" frame="true" align="right" spinBox="true"
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'''NMR SOLUTION STRUCTURE OF CALCIUM-LOADED CALMODULIN CARBOXY-TERMINAL DOMAIN'''<br />
'''NMR SOLUTION STRUCTURE OF CALCIUM-LOADED CALMODULIN CARBOXY-TERMINAL DOMAIN'''<br />
==Overview==
==Overview==
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We have determined the solution structures of the apo and (Ca2+)2 forms of, the carboxy-terminal domain of calmodulin using multidimensional, heteronuclear nuclear magnetic resonance spectroscopy. The results show, that both forms adopt well-defined structures with essentially equal, secondary structure. A comparison of the structures of the two forms shows, that Ca2+ binding causes major rearrangements of the secondary structure, elements with changes in inter-residue distances of up to 15 A and, exposure of the hydrophobic interior of the four-helix bundle. Comparisons, with previously determined high-resolution X-ray structures and models of, calmodulin indicate that this domain is structurally autonomous.
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We have determined the solution structures of the apo and (Ca2+)2 forms of the carboxy-terminal domain of calmodulin using multidimensional heteronuclear nuclear magnetic resonance spectroscopy. The results show that both forms adopt well-defined structures with essentially equal secondary structure. A comparison of the structures of the two forms shows that Ca2+ binding causes major rearrangements of the secondary structure elements with changes in inter-residue distances of up to 15 A and exposure of the hydrophobic interior of the four-helix bundle. Comparisons with previously determined high-resolution X-ray structures and models of calmodulin indicate that this domain is structurally autonomous.
==About this Structure==
==About this Structure==
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1CMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CMG OCA].
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1CMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CMG OCA].
==Reference==
==Reference==
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[[Category: Drakenberg, T.]]
[[Category: Drakenberg, T.]]
[[Category: Evenas, J.]]
[[Category: Evenas, J.]]
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[[Category: Finn, B.E.]]
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[[Category: Finn, B E.]]
[[Category: Forsen, S.]]
[[Category: Forsen, S.]]
[[Category: Thulin, E.]]
[[Category: Thulin, E.]]
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[[Category: Waltho, J.P.]]
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[[Category: Waltho, J P.]]
[[Category: calcium-binding protein]]
[[Category: calcium-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:34:53 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:31 2008''

Revision as of 10:07, 21 February 2008


1cmg

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NMR SOLUTION STRUCTURE OF CALCIUM-LOADED CALMODULIN CARBOXY-TERMINAL DOMAIN

Overview

We have determined the solution structures of the apo and (Ca2+)2 forms of the carboxy-terminal domain of calmodulin using multidimensional heteronuclear nuclear magnetic resonance spectroscopy. The results show that both forms adopt well-defined structures with essentially equal secondary structure. A comparison of the structures of the two forms shows that Ca2+ binding causes major rearrangements of the secondary structure elements with changes in inter-residue distances of up to 15 A and exposure of the hydrophobic interior of the four-helix bundle. Comparisons with previously determined high-resolution X-ray structures and models of calmodulin indicate that this domain is structurally autonomous.

About this Structure

1CMG is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Calcium-induced structural changes and domain autonomy in calmodulin., Finn BE, Evenas J, Drakenberg T, Waltho JP, Thulin E, Forsen S, Nat Struct Biol. 1995 Sep;2(9):777-83. PMID:7552749

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