1cr4
From Proteopedia
(New page: 200px<br /><applet load="1cr4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cr4, resolution 2.50Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1cr4.gif|left|200px]]<br /><applet load="1cr4" size=" | + | [[Image:1cr4.gif|left|200px]]<br /><applet load="1cr4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cr4, resolution 2.50Å" /> | caption="1cr4, resolution 2.50Å" /> | ||
'''CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP'''<br /> | '''CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Helicases that unwind DNA at the replication fork are ring-shaped | + | Helicases that unwind DNA at the replication fork are ring-shaped oligomeric enzymes that move along one strand of a DNA duplex and catalyze the displacement of the complementary strand in a reaction that is coupled to nucleotide hydrolysis. The helicase domain of the replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that resembles the Escherichia coli RecA protein, an ATP-dependent DNA strand exchange factor. When viewed in projection along the helical axis of the crystals, six protomers of the T7 helicase domain resemble the hexameric rings seen in electron microscopic images of the intact T7 helicase-primase. Nucleotides bind at the interface between pairs of adjacent subunits where an arginine is near the gamma-phosphate of the nucleotide in trans. The bound nucleotide stabilizes the folded conformation of a DNA-binding motif located near the center of the ring. These and other observations suggest how conformational changes are coupled to DNA unwinding activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1CR4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with SO4 and TYD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1CR4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TYD:'>TYD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR4 OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
[[Category: Guo, S.]] | [[Category: Guo, S.]] | ||
| - | [[Category: Richardson, C | + | [[Category: Richardson, C C.]] |
| - | [[Category: Sawaya, M | + | [[Category: Sawaya, M R.]] |
[[Category: Tabor, S.]] | [[Category: Tabor, S.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| Line 23: | Line 23: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:52 2008'' |
Revision as of 10:08, 21 February 2008
|
CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP
Overview
Helicases that unwind DNA at the replication fork are ring-shaped oligomeric enzymes that move along one strand of a DNA duplex and catalyze the displacement of the complementary strand in a reaction that is coupled to nucleotide hydrolysis. The helicase domain of the replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that resembles the Escherichia coli RecA protein, an ATP-dependent DNA strand exchange factor. When viewed in projection along the helical axis of the crystals, six protomers of the T7 helicase domain resemble the hexameric rings seen in electron microscopic images of the intact T7 helicase-primase. Nucleotides bind at the interface between pairs of adjacent subunits where an arginine is near the gamma-phosphate of the nucleotide in trans. The bound nucleotide stabilizes the folded conformation of a DNA-binding motif located near the center of the ring. These and other observations suggest how conformational changes are coupled to DNA unwinding activity.
About this Structure
1CR4 is a Single protein structure of sequence from Bacteriophage t7 with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7., Sawaya MR, Guo S, Tabor S, Richardson CC, Ellenberger T, Cell. 1999 Oct 15;99(2):167-77. PMID:10535735
Page seeded by OCA on Thu Feb 21 12:08:52 2008
