1cvu
From Proteopedia
(New page: 200px<br /><applet load="1cvu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cvu, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1cvu.gif|left|200px]]<br /><applet load="1cvu" size=" | + | [[Image:1cvu.gif|left|200px]]<br /><applet load="1cvu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cvu, resolution 2.4Å" /> | caption="1cvu, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF ARACHIDONIC ACID BOUND TO THE CYCLOOXYGENASE ACTIVE SITE OF COX-2'''<br /> | '''CRYSTAL STRUCTURE OF ARACHIDONIC ACID BOUND TO THE CYCLOOXYGENASE ACTIVE SITE OF COX-2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cyclooxygenases are bifunctional enzymes that catalyse the first committed | + | Cyclooxygenases are bifunctional enzymes that catalyse the first committed step in the synthesis of prostaglandins, thromboxanes and other eicosanoids. The two known cyclooxygenases isoforms share a high degree of amino-acid sequence similarity, structural topology and an identical catalytic mechanism. Cyclooxygenase enzymes catalyse two sequential reactions in spatially distinct, but mechanistically coupled active sites. The initial cyclooxygenase reaction converts arachidonic acid (which is achiral) to prostaglandin G2 (which has five chiral centres). The subsequent peroxidase reaction reduces prostaglandin G2 to prostaglandin H2. Here we report the co-crystal structures of murine apo-cyclooxygenase-2 in complex with arachidonic acid and prostaglandin. These structures suggest the molecular basis for the stereospecificity of prostaglandin G2 synthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1CVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG, BOG and ACD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-endoperoxide_synthase Prostaglandin-endoperoxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.1 1.14.99.1] Full crystallographic information is available from [http:// | + | 1CVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=BOG:'>BOG</scene> and <scene name='pdbligand=ACD:'>ACD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Prostaglandin-endoperoxide_synthase Prostaglandin-endoperoxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.1 1.14.99.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Prostaglandin-endoperoxide synthase]] | [[Category: Prostaglandin-endoperoxide synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Gierse, J | + | [[Category: Gierse, J K.]] |
| - | [[Category: Goodwin, D | + | [[Category: Goodwin, D C.]] |
| - | [[Category: Kiefer, J | + | [[Category: Kiefer, J R.]] |
| - | [[Category: Kurumbail, R | + | [[Category: Kurumbail, R G.]] |
| - | [[Category: Marnett, L | + | [[Category: Marnett, L J.]] |
| - | [[Category: Moreland, K | + | [[Category: Moreland, K T.]] |
| - | [[Category: Pawlitz, J | + | [[Category: Pawlitz, J L.]] |
| - | [[Category: Rowlinson, S | + | [[Category: Rowlinson, S W.]] |
| - | [[Category: Stallings, W | + | [[Category: Stallings, W C.]] |
| - | [[Category: Stegeman, R | + | [[Category: Stegeman, R A.]] |
| - | [[Category: Stevens, A | + | [[Category: Stevens, A M.]] |
[[Category: ACD]] | [[Category: ACD]] | ||
[[Category: BOG]] | [[Category: BOG]] | ||
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[[Category: prostaglandin]] | [[Category: prostaglandin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:16 2008'' |
Revision as of 10:10, 21 February 2008
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CRYSTAL STRUCTURE OF ARACHIDONIC ACID BOUND TO THE CYCLOOXYGENASE ACTIVE SITE OF COX-2
Overview
Cyclooxygenases are bifunctional enzymes that catalyse the first committed step in the synthesis of prostaglandins, thromboxanes and other eicosanoids. The two known cyclooxygenases isoforms share a high degree of amino-acid sequence similarity, structural topology and an identical catalytic mechanism. Cyclooxygenase enzymes catalyse two sequential reactions in spatially distinct, but mechanistically coupled active sites. The initial cyclooxygenase reaction converts arachidonic acid (which is achiral) to prostaglandin G2 (which has five chiral centres). The subsequent peroxidase reaction reduces prostaglandin G2 to prostaglandin H2. Here we report the co-crystal structures of murine apo-cyclooxygenase-2 in complex with arachidonic acid and prostaglandin. These structures suggest the molecular basis for the stereospecificity of prostaglandin G2 synthesis.
About this Structure
1CVU is a Single protein structure of sequence from Mus musculus with , and as ligands. Active as Prostaglandin-endoperoxide synthase, with EC number 1.14.99.1 Full crystallographic information is available from OCA.
Reference
Structural insights into the stereochemistry of the cyclooxygenase reaction., Kiefer JR, Pawlitz JL, Moreland KT, Stegeman RA, Hood WF, Gierse JK, Stevens AM, Goodwin DC, Rowlinson SW, Marnett LJ, Stallings WC, Kurumbail RG, Nature. 2000 May 4;405(6782):97-101. PMID:10811226
Page seeded by OCA on Thu Feb 21 12:10:16 2008
Categories: Mus musculus | Prostaglandin-endoperoxide synthase | Single protein | Gierse, J K. | Goodwin, D C. | Kiefer, J R. | Kurumbail, R G. | Marnett, L J. | Moreland, K T. | Pawlitz, J L. | Rowlinson, S W. | Stallings, W C. | Stegeman, R A. | Stevens, A M. | ACD | BOG | NAG | Arachidonate | Cox-2 | Cyclooxygenase | Endoperoxide | Prostaglandin
