1cxc
From Proteopedia
(New page: 200px<br /><applet load="1cxc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cxc, resolution 1.6Å" /> '''CRYSTALLIZATION AND X...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size=" | + | [[Image:1cxc.jpg|left|200px]]<br /><applet load="1cxc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cxc, resolution 1.6Å" /> | caption="1cxc, resolution 1.6Å" /> | ||
'''CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS'''<br /> | '''CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cytochrome c(2) serves as the secondary electron donor that reduces the | + | Cytochrome c(2) serves as the secondary electron donor that reduces the photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria. Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in three different forms. At high ionic strength, crystals of a hexagonal space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The three-dimensional structures of the cytochrome in all three crystal forms have been determined by X-ray diffraction at resolutions of 2.20 A (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most significant difference observed was the binding of an imidazole molecule to the iron atom of the heme group in the hexagonal structure. This binding displaces the sulfur atom of Met l00, which forms the axial ligand in the triclinic and tetragonal structures. |
==About this Structure== | ==About this Structure== | ||
| - | 1CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXC OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Allen, J | + | [[Category: Allen, J P.]] |
| - | [[Category: Axelrod, H | + | [[Category: Axelrod, H L.]] |
| - | [[Category: Chirino, A | + | [[Category: Chirino, A J.]] |
| - | [[Category: Day, M | + | [[Category: Day, M W.]] |
[[Category: Feher, G.]] | [[Category: Feher, G.]] | ||
| - | [[Category: Hsu, B | + | [[Category: Hsu, B T.]] |
| - | [[Category: Rees, D | + | [[Category: Rees, D C.]] |
[[Category: HEM]] | [[Category: HEM]] | ||
[[Category: electron transport (cytochrome)]] | [[Category: electron transport (cytochrome)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:44 2008'' |
Revision as of 10:10, 21 February 2008
|
CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS
Overview
Cytochrome c(2) serves as the secondary electron donor that reduces the photo-oxidized bacteriochlorophyll dimer in photosynthetic bacteria. Cytochrome c(2) from Rhodobacter sphaeroides has been crystallized in three different forms. At high ionic strength, crystals of a hexagonal space group (P6(1)22) were obtained, while at low ionic strength, triclinic (P1) and tetragonal (P4(1)2(1)2) crystals were formed. The three-dimensional structures of the cytochrome in all three crystal forms have been determined by X-ray diffraction at resolutions of 2.20 A (hexagonal), 1.95 A, (triclinic) and 1.53 A (tetragonal). The most significant difference observed was the binding of an imidazole molecule to the iron atom of the heme group in the hexagonal structure. This binding displaces the sulfur atom of Met l00, which forms the axial ligand in the triclinic and tetragonal structures.
About this Structure
1CXC is a Single protein structure of sequence from Rhodobacter sphaeroides with as ligand. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray structure determination of cytochrome c2 from Rhodobacter sphaeroides in three crystal forms., Axelrod HL, Feher G, Allen JP, Chirino AJ, Day MW, Hsu BT, Rees DC, Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):596-602. PMID:15299423
Page seeded by OCA on Thu Feb 21 12:10:44 2008
