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1e6r
From Proteopedia
(New page: 200px<br /> <applet load="1e6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e6r, resolution 2.50Å" /> '''CHITINASE B FROM SE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E6R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with SO4 and AMI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6R OCA]]. | + | 1E6R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with SO4 and AMI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AMA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6R OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: inhibitor allosamidin]] | [[Category: inhibitor allosamidin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:39:54 2007'' |
Revision as of 10:35, 30 October 2007
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CHITINASE B FROM SERRATIA MARCESCENS WILDTYPE IN COMPLEX WITH INHIBITOR ALLOSAMIDIN
Overview
Chitinase B (ChiB) from Serratia marcescens is a family 18 exo-chitinase, whose catalytic domain has a TIM-barrel fold with a tunnel-shaped active, site. We have solved structures of three ChiB complexes that reveal, details of substrate binding, substrate-assisted catalysis, and product, displacement. The structure of an inactive ChiB mutant (E144Q) complexed, with a pentameric substrate (binding in subsites -2 to +3) shows closure, of the "roof" of the active site tunnel. It also shows that the sugar in, the -1 position is distorted to a boat conformation, thus providing, structural evidence in support of a previously proposed catalytic, mechanism. The structures of the active enzyme complexed to allosamidin, (an analogue of a proposed reaction intermediate) and of the active enzyme, ... [(full description)]
About this Structure
1E6R is a [Single protein] structure of sequence from [Serratia marcescens] with SO4 and AMI as [ligands]. Structure known Active Site: AMA. Full crystallographic information is available from [OCA].
Reference
Structural insights into the catalytic mechanism of a family 18 exo-chitinase., van Aalten DM, Komander D, Synstad B, Gaseidnes S, Peter MG, Eijsink VG, Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8979-84. PMID:11481469
Page seeded by OCA on Tue Oct 30 12:39:54 2007
