1cxv
From Proteopedia
(New page: 200px<br /><applet load="1cxv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cxv, resolution 2.00Å" /> '''STRUCTURE OF RECOMBI...) |
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| - | [[Image:1cxv.gif|left|200px]]<br /><applet load="1cxv" size=" | + | [[Image:1cxv.gif|left|200px]]<br /><applet load="1cxv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cxv, resolution 2.00Å" /> | caption="1cxv, resolution 2.00Å" /> | ||
'''STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)'''<br /> | '''STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The matrix metalloproteinases are crucial in the physiological and | + | The matrix metalloproteinases are crucial in the physiological and pathological degradation of the mammalian extracellular matrix, including breast tumours, and osteoarthritic cartilage. These enzymes are classified according to their matrix substrate specificity. Collagenase-3 (MMP-13) is a member of this family and preferentially cleaves type II collagen, cartilage, fibronectin and aggrecan. Collagenase-3 is normally expressed in hypertrophic chondrocytes, periosteal cells, and osteoblasts during bone development. The structure of the catalytic domain of recombinant mouse collagenase-3, complexed to the hydroxamate inhibitor (RS-113456), is reported at 2.0 A resolution. Molecular replacement and weak phasing information from a single derivative determined the structure. Neither molecular replacement nor derivative methods had a sufficient radius of convergence to yield a refinable structure. The structure illuminates the atomic zinc ion interactions with functional groups in the active site, emphasizing zinc ligation and the very voluminous hydrophobic P1' group for the inhibitor potency. The structure provides insight into the specificity of this enzyme, facilitating design of specific inhibitors to target various diseases. |
==About this Structure== | ==About this Structure== | ||
| - | 1CXV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN, CA and CBP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1CXV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CBP:'>CBP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lemaitre, V.]] | [[Category: Lemaitre, V.]] | ||
[[Category: Meyer, E.]] | [[Category: Meyer, E.]] | ||
| - | [[Category: Meyer, E | + | [[Category: Meyer, E F.]] |
| - | [[Category: Swanson, S | + | [[Category: Swanson, S M.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: CBP]] | [[Category: CBP]] | ||
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[[Category: metalloprotease]] | [[Category: metalloprotease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:49 2008'' |
Revision as of 10:10, 21 February 2008
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STRUCTURE OF RECOMBINANT MOUSE COLLAGENASE-3 (MMP-13)
Overview
The matrix metalloproteinases are crucial in the physiological and pathological degradation of the mammalian extracellular matrix, including breast tumours, and osteoarthritic cartilage. These enzymes are classified according to their matrix substrate specificity. Collagenase-3 (MMP-13) is a member of this family and preferentially cleaves type II collagen, cartilage, fibronectin and aggrecan. Collagenase-3 is normally expressed in hypertrophic chondrocytes, periosteal cells, and osteoblasts during bone development. The structure of the catalytic domain of recombinant mouse collagenase-3, complexed to the hydroxamate inhibitor (RS-113456), is reported at 2.0 A resolution. Molecular replacement and weak phasing information from a single derivative determined the structure. Neither molecular replacement nor derivative methods had a sufficient radius of convergence to yield a refinable structure. The structure illuminates the atomic zinc ion interactions with functional groups in the active site, emphasizing zinc ligation and the very voluminous hydrophobic P1' group for the inhibitor potency. The structure provides insight into the specificity of this enzyme, facilitating design of specific inhibitors to target various diseases.
About this Structure
1CXV is a Single protein structure of sequence from Mus musculus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of recombinant mouse collagenase-3 (MMP-13)., Botos I, Meyer E, Swanson SM, Lemaitre V, Eeckhout Y, Meyer EF, J Mol Biol. 1999 Oct 1;292(4):837-44. PMID:10525409
Page seeded by OCA on Thu Feb 21 12:10:49 2008
Categories: Mus musculus | Single protein | Botos, I. | Eeckhout, Y. | Lemaitre, V. | Meyer, E. | Meyer, E F. | Swanson, S M. | CA | CBP | ZN | Collagen degradation | Glycoprotein | Metalloprotease
